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Activation of human B lymphocytes. III. Concanavalin A-induced generation of suppressor cells of the plaque-forming cell response of normal human B lymphocytes.

Publication ,  Journal Article
Haynes, BF; Fauci, AS
Published in: J Immunol
June 1977

Con A-induced suppression of the direct PFC response to polyclonal stimulation in human B cells has been described. Two types of experiments are presented. First, Con A was added directly to PWM-stimulated PB or tonsil cells resulting in a dose-dependent suppression of the PFC response, with maximal suppression occurring at a Con A concentration of 10 mug/ml. This suppression is completely removed by the simultaneous addition of alphaMM to the cultures. Secondly, Con A stimulation of tonsil or PB lymphocytes generated a population of cells which when added to autologous lymphocyte cultures induced a marked and reproducible suppression of the PFC response. The generation of suppressor cells is dependent on cell division and is blocked by alpha MM. Once generated the process of suppression is indpendent of the presence of Con A itself and is mediated by an activated lymphocyte population. These studies demonstrate a simple and reproducible model for the generation of a population of suppressor cells capable of inhibiting the direct PFC response to PWM-induced polyclonal activation of normal human B lymphocytes.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1977

Volume

118

Issue

6

Start / End Page

2281 / 2287

Location

United States

Related Subject Headings

  • Methylmannosides
  • Lymphocyte Activation
  • Lectins
  • Immunosuppression Therapy
  • Immunology
  • Immunity, Cellular
  • Humans
  • Hemolytic Plaque Technique
  • Dose-Response Relationship, Immunologic
  • Concanavalin A
 

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1977

Volume

118

Issue

6

Start / End Page

2281 / 2287

Location

United States

Related Subject Headings

  • Methylmannosides
  • Lymphocyte Activation
  • Lectins
  • Immunosuppression Therapy
  • Immunology
  • Immunity, Cellular
  • Humans
  • Hemolytic Plaque Technique
  • Dose-Response Relationship, Immunologic
  • Concanavalin A