Skip to main content

Human erythrocyte antigens: II. The In(Lu) gene regulates expression of an antigen on an 80-kilodalton protein of human erythrocytes.

Publication ,  Journal Article
Telen, MJ; Palker, TJ; Haynes, BF
Published in: Blood
September 1984

We have previously shown that a murine monoclonal antibody (A3D8) identifies a human erythrocyte protein antigen whose expression is regulated by the Lutheran inhibitor [In(Lu)] gene. In the present study, we demonstrated by immunoprecipitation and Western blot techniques that the antigen defined by A3D8 was on an 80-kD erythrocyte membrane protein. A second 170-kD protein was coprecipitated with the 80-kD protein but failed to show antigen activity by Western blot analysis. The 170-kD protein, when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in two dimensions, was composed of 50- and 30-kD disulfide-linked subunits. In(Lu) Lu[a-b-) erythrocytes differed from Lu(a+b+) or Lu(a-b+) erythrocytes in that In(Lu) deoxycholate erythrocyte membrane extracts contained trace amounts of immunoprecipitable 80-kD protein compared with detergent-solubilized erythrocyte membrane extracts prepared from Lu(a+b+) or Lu(a-b+) subjects.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

September 1984

Volume

64

Issue

3

Start / End Page

599 / 606

Location

United States

Related Subject Headings

  • Sodium Dodecyl Sulfate
  • Molecular Weight
  • Mice, Inbred BALB C
  • Mice
  • Membrane Proteins
  • Lutheran Blood-Group System
  • Immunology
  • Humans
  • Genes, Regulator
  • Erythrocyte Membrane
 

Published In

Blood

ISSN

0006-4971

Publication Date

September 1984

Volume

64

Issue

3

Start / End Page

599 / 606

Location

United States

Related Subject Headings

  • Sodium Dodecyl Sulfate
  • Molecular Weight
  • Mice, Inbred BALB C
  • Mice
  • Membrane Proteins
  • Lutheran Blood-Group System
  • Immunology
  • Humans
  • Genes, Regulator
  • Erythrocyte Membrane