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Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV(89.6P) challenge in rhesus macaques.

Publication ,  Journal Article
Chong, S-Y; Egan, MA; Kutzler, MA; Megati, S; Masood, A; Roopchard, V; Garcia-Hand, D; Montefiori, DC; Quiroz, J; Rosati, M; Schadeck, EB ...
Published in: Vaccine
June 21, 2007

Plasmid-based IL-12 has been demonstrated to successfully enhance the immunogenicity of DNA vaccines, thus enabling a reduction of the amount of DNA required for immunization. IL-15 is thought to affect the maintenance and enhance effector function of CD8(+) memory T cells. Since the ability to elicit a long-term memory response is a desirable attribute of a prophylactic vaccine, we sought to evaluate the ability of these plasmid-based cytokines to serve as vaccine adjuvants in rhesus macaques. Macaques were immunized with plasmid DNA encoding SIVgag in combination with plasmid IL-12, IL-15, or a combination of IL-12 and IL-15. The plasmid-based cytokines were monitored for their ability to augment SIVgag-specific cellular and humoral immune responses and to alter the clinical outcome following pathogenic SHIV(89.6P) challenge. Macaques receiving SIVgag pDNA in combination with plasmid IL-12 alone, or in combination with plasmid IL-12 and IL-15, demonstrated significantly elevated cell-mediated and humoral immune responses resulting in an improved clinical outcome following virus challenge compared to macaques receiving SIVgag pDNA alone. Macaques receiving SIVgag pDNA in combination with plasmid IL-15 alone demonstrated minor increases in cell-mediated and humoral immune responses, however, the clinical outcome following virus challenge was not improved. These results have important implications for the continued development of plasmid DNA vaccines for the prevention of HIV-1 infection.

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Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

June 21, 2007

Volume

25

Issue

26

Start / End Page

4967 / 4982

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Plasmids
  • Neutralization Tests
  • Male
  • Macaca mulatta
  • Interleukin-15
  • Interleukin-12
 

Citation

APA
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MLA
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Chong, S.-Y., Egan, M. A., Kutzler, M. A., Megati, S., Masood, A., Roopchard, V., … Israel, Z. R. (2007). Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV(89.6P) challenge in rhesus macaques. Vaccine, 25(26), 4967–4982. https://doi.org/10.1016/j.vaccine.2006.11.070
Chong, Siew-Yen, Michael A. Egan, Michele A. Kutzler, Shakuntala Megati, Amjed Masood, Vidia Roopchard, Dorys Garcia-Hand, et al. “Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV(89.6P) challenge in rhesus macaques.Vaccine 25, no. 26 (June 21, 2007): 4967–82. https://doi.org/10.1016/j.vaccine.2006.11.070.
Chong S-Y, Egan MA, Kutzler MA, Megati S, Masood A, Roopchard V, Garcia-Hand D, Montefiori DC, Quiroz J, Rosati M, Schadeck EB, Boyer JD, Pavlakis GN, Weiner DB, Sidhu M, Eldridge JH, Israel ZR. Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV(89.6P) challenge in rhesus macaques. Vaccine. 2007 Jun 21;25(26):4967–4982.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

June 21, 2007

Volume

25

Issue

26

Start / End Page

4967 / 4982

Location

Netherlands

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Plasmids
  • Neutralization Tests
  • Male
  • Macaca mulatta
  • Interleukin-15
  • Interleukin-12