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Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles.

Publication ,  Journal Article
Mossman, SP; Pierce, CC; Watson, AJ; Robertson, MN; Montefiori, DC; Kuller, L; Richardson, BA; Bradshaw, JD; Munn, RJ; Hu, S-L; Greenberg, PD ...
Published in: AIDS Res Hum Retroviruses
April 2004

We utilized SIV(mne) infection of Macaca fascicularis to assess the efficacy of DNA vaccination alone, and as a priming agent in combination with subunit protein boosts. All SIV(mne) structural and regulatory genes were expressed using the human cytomegalovirus Immediate Early-1 promoter in plasmids that directed the formation of virus-like particles in vitro. Macaques (n = 4) were immunized intradermally and intramuscularly four times over 36 weeks with 3 mg plasmid DNA. A second group (n = 4) received two DNA priming inoculations followed by two intramuscular boosts consisting of 250 microg recombinant Env gp160 and 250 microg recombinant Gag-Pol particles in MF-59 adjuvant. These regimens elicited modest cellular immunity prior to challenge. Humoral immune responses to Env gp160 were elicited and sustained by both vaccine protocols, and as expected antibody titers were higher in the protein subunit-boosted animals. Neutralizing antibodies prior to challenge were measurable in two of four subunit-boosted macaques. The two vaccine regimens elicited comparable helper T cell responses at the time of challenge. Vaccinees and mock-immunized controls (n = 4) were challenged intrarectally at week 38 with uncloned SIV(mne). Following challenge all macaques became infected, but both vaccine regimens resulted in reduced peak virus loads (p = 0.07) and significantly improved maintenance of peripheral CD4(+) T cell counts postchallenge (p = 0.007, DNA alone and p = 0.01, all vaccinees). There was no significant difference between the two vaccine groups in levels of plasma viremia or maintenance of CD4(+) T cell counts postchallenge.

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Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

April 2004

Volume

20

Issue

4

Start / End Page

425 / 434

Location

United States

Related Subject Headings

  • Virology
  • Viral Load
  • Vaccines, Subunit
  • Vaccines, DNA
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • RNA, Viral
  • Proviruses
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mossman, S. P., Pierce, C. C., Watson, A. J., Robertson, M. N., Montefiori, D. C., Kuller, L., … Haigwood, N. L. (2004). Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles. AIDS Res Hum Retroviruses, 20(4), 425–434. https://doi.org/10.1089/088922204323048177
Mossman, Sally P., Christopher C. Pierce, Andrew J. Watson, Michael N. Robertson, David C. Montefiori, Larene Kuller, Barbra A. Richardson, et al. “Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles.AIDS Res Hum Retroviruses 20, no. 4 (April 2004): 425–34. https://doi.org/10.1089/088922204323048177.
Mossman SP, Pierce CC, Watson AJ, Robertson MN, Montefiori DC, Kuller L, et al. Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles. AIDS Res Hum Retroviruses. 2004 Apr;20(4):425–34.
Mossman, Sally P., et al. “Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles.AIDS Res Hum Retroviruses, vol. 20, no. 4, Apr. 2004, pp. 425–34. Pubmed, doi:10.1089/088922204323048177.
Mossman SP, Pierce CC, Watson AJ, Robertson MN, Montefiori DC, Kuller L, Richardson BA, Bradshaw JD, Munn RJ, Hu S-L, Greenberg PD, Benveniste RE, Haigwood NL. Protective immunity to SIV challenge elicited by vaccination of macaques with multigenic DNA vaccines producing virus-like particles. AIDS Res Hum Retroviruses. 2004 Apr;20(4):425–434.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

April 2004

Volume

20

Issue

4

Start / End Page

425 / 434

Location

United States

Related Subject Headings

  • Virology
  • Viral Load
  • Vaccines, Subunit
  • Vaccines, DNA
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • RNA, Viral
  • Proviruses