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Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6).

Publication ,  Journal Article
Ambrose, Z; Thompson, J; Larsen, K; Kuller, L; Panicali, DL; Clements, JD; Agy, M; Montefiori, DC; Hu, SL; Bosch, ML
Published in: Virology
March 30, 2003

Although most HIV-1 infections worldwide result from heterosexual transmission, most vaccine candidates have focused on induction of systemic immunity and protection. We hypothesized that combining systemic priming with mucosal boosting would induce mucosal immunity that would protect from intravaginal challenge. Macaques were primed systemically with recombinant vaccinia viruses and boosted mucosally using inactivated SHIV(89.6) plus adjuvant. Other animals received protein boosts with adjuvant alone. Priming and boosting induced antiviral IgG and IgA antibodies. Such antibodies were induced to a lesser degree in animals receiving boosts alone. Anti-SHIV T cell responses were induced only in the prime-boost animals. Immunized animals and controls were challenged intravaginally with SHIV(89.6) and significant reductions in proviral and viral RNA loads were observed in the prime-boost animals. The boost-only animals did not have significant viral load reductions. These data suggest that cellular immunity was required for protection from intravaginal challenge. This immunization regimen provides a promising lead for vaccine development.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 30, 2003

Volume

308

Issue

1

Start / End Page

178 / 190

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Load
  • Vaccines, Synthetic
  • T-Lymphocytes
  • RNA, Viral
  • Proviruses
  • Macaca nemestrina
  • Lentiviruses, Primate
  • Immunoglobulin G
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ambrose, Z., Thompson, J., Larsen, K., Kuller, L., Panicali, D. L., Clements, J. D., … Bosch, M. L. (2003). Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6). Virology, 308(1), 178–190. https://doi.org/10.1016/s0042-6822(03)00027-8
Ambrose, Zandrea, Jannelle Thompson, Kay Larsen, Larene Kuller, Dennis L. Panicali, John D. Clements, Michael Agy, David C. Montefiori, Shiu Lok Hu, and Marnix L. Bosch. “Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6).Virology 308, no. 1 (March 30, 2003): 178–90. https://doi.org/10.1016/s0042-6822(03)00027-8.
Ambrose Z, Thompson J, Larsen K, Kuller L, Panicali DL, Clements JD, et al. Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6). Virology. 2003 Mar 30;308(1):178–90.
Ambrose, Zandrea, et al. “Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6).Virology, vol. 308, no. 1, Mar. 2003, pp. 178–90. Pubmed, doi:10.1016/s0042-6822(03)00027-8.
Ambrose Z, Thompson J, Larsen K, Kuller L, Panicali DL, Clements JD, Agy M, Montefiori DC, Hu SL, Bosch ML. Evidence for immune-mediated reduction of viral replication in Macaca nemestrina mucosally immunized with inactivated SHIV(89.6). Virology. 2003 Mar 30;308(1):178–190.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 30, 2003

Volume

308

Issue

1

Start / End Page

178 / 190

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Load
  • Vaccines, Synthetic
  • T-Lymphocytes
  • RNA, Viral
  • Proviruses
  • Macaca nemestrina
  • Lentiviruses, Primate
  • Immunoglobulin G