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Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.

Publication ,  Journal Article
Prichard, HL; Schroeder, T; Reichert, WM; Klitzman, B
Published in: J Diabetes Sci Technol
September 1, 2010

BACKGROUND: The bioluminescence technique was used to quantify the local glucose concentration in the tissue surrounding subcutaneously implanted polyurethane material and surrounding glucose sensors. In addition, some implants were coated with a single layer of adipose-derived stromal cells (ASCs) because these cells improve the wound-healing response around biomaterials. METHODS: Control and ASC-coated implants were implanted subcutaneously in rats for 1 or 8 weeks (polyurethane) or for 1 week only (glucose sensors). Tissue biopsies adjacent to the implant were immediately frozen at the time of explant. Cryosections were assayed for glucose concentration profile using the bioluminescence technique. RESULTS: For the polyurethane samples, no significant differences in glucose concentration within 100 μm of the implant surface were found between bare and ASC-coated implants at 1 or 8 weeks. A glucose concentration gradient was demonstrated around the glucose sensors. For all sensors, the minimum glucose concentration of approximately 4 mM was found at the implant surface and increased with distance from the sensor surface until the glucose concentration peaked at approximately 7 mM at 100 μm. Then the glucose concentration decreased to 5.5-6.5 mM more than 100 μmm from the surface. CONCLUSIONS: The ASC attachment to polyurethane and to glucose sensors did not change the glucose profiles in the tissue surrounding the implants. Although most glucose sensors incorporate a diffusion barrier to reduce the gradient of glucose and oxygen in the tissue, it is typically assumed that there is no steep glucose gradient around the sensors. However, a glucose gradient was observed around the sensors. A more complete understanding of glucose transport and concentration gradients around sensors is critical.

Duke Scholars

Published In

J Diabetes Sci Technol

DOI

EISSN

1932-2968

Publication Date

September 1, 2010

Volume

4

Issue

5

Start / End Page

1055 / 1062

Location

United States

Related Subject Headings

  • Stromal Cells
  • Skin
  • Rats, Inbred Lew
  • Rats
  • Polyurethanes
  • Oxygen
  • Models, Animal
  • Male
  • Luminescence
  • Implants, Experimental
 

Citation

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Chicago
ICMJE
MLA
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Prichard, H. L., Schroeder, T., Reichert, W. M., & Klitzman, B. (2010). Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants. J Diabetes Sci Technol, 4(5), 1055–1062. https://doi.org/10.1177/193229681000400504
Prichard, Heather L., Thies Schroeder, William M. Reichert, and Bruce Klitzman. “Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.J Diabetes Sci Technol 4, no. 5 (September 1, 2010): 1055–62. https://doi.org/10.1177/193229681000400504.
Prichard HL, Schroeder T, Reichert WM, Klitzman B. Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants. J Diabetes Sci Technol. 2010 Sep 1;4(5):1055–62.
Prichard, Heather L., et al. “Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants.J Diabetes Sci Technol, vol. 4, no. 5, Sept. 2010, pp. 1055–62. Pubmed, doi:10.1177/193229681000400504.
Prichard HL, Schroeder T, Reichert WM, Klitzman B. Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants. J Diabetes Sci Technol. 2010 Sep 1;4(5):1055–1062.
Journal cover image

Published In

J Diabetes Sci Technol

DOI

EISSN

1932-2968

Publication Date

September 1, 2010

Volume

4

Issue

5

Start / End Page

1055 / 1062

Location

United States

Related Subject Headings

  • Stromal Cells
  • Skin
  • Rats, Inbred Lew
  • Rats
  • Polyurethanes
  • Oxygen
  • Models, Animal
  • Male
  • Luminescence
  • Implants, Experimental