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In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody.

Publication ,  Journal Article
Stuhlmiller, GM; Sullivan, DC; Vervaert, CE; Croker, BP; Harris, CC; Seigler, HF
Published in: Ann Surg
November 1981

Specific in vivo localization of antibodies reactive with human melanoma cell membrane tumor associated antigens (TAA) has been attempted using congenitally athymic nude mice bearing subcutaneous human melanoma tumor xenografts as the experimental model. IgG fractions were prepared from each of several immune and control sera. Antimelanoma antibody sources included human alloantibody obtained from melanoma patients immunized against allogeneic melanoma cells, a monkey antiserum raised by immunization against a single human melanoma cell line, and a murine monoclonal antimelanoma antibody-secreting hybridoma cell line. Localization of these radiolabeled antibodies and of control IgG preparations to tumor tissue was determined by whole body scintigraphy and by differential tissue counting. Compared with the different control IgG preparations, each of the antimelanoma IgG preparations exhibited significant specific accumulation within the melanoma tissue. However, variation existed in the ability of each antimelanoma IgG to tumor preparation to localize despite attempts to control model parameters such as tumor source, in vivo passage number and mass. This variation appears to reflect basic biologic differences between tumors in different animals and possibly differences in the antigen-binding capacities of each IgG preparation following radioiodination. This technique for tumor localization is very promising and has obvious potential for clinical application.

Duke Scholars

Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

November 1981

Volume

194

Issue

5

Start / End Page

592 / 601

Location

United States

Related Subject Headings

  • Surgery
  • Radionuclide Imaging
  • Neoplasms, Experimental
  • Neoplasm Transplantation
  • Neoplasm Proteins
  • Mice, Nude
  • Mice
  • Melanoma-Specific Antigens
  • Melanoma
  • Macaca mulatta
 

Citation

APA
Chicago
ICMJE
MLA
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Stuhlmiller, G. M., Sullivan, D. C., Vervaert, C. E., Croker, B. P., Harris, C. C., & Seigler, H. F. (1981). In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody. Ann Surg, 194(5), 592–601. https://doi.org/10.1097/00000658-198111000-00007
Stuhlmiller, G. M., D. C. Sullivan, C. E. Vervaert, B. P. Croker, C. C. Harris, and H. F. Seigler. “In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody.Ann Surg 194, no. 5 (November 1981): 592–601. https://doi.org/10.1097/00000658-198111000-00007.
Stuhlmiller GM, Sullivan DC, Vervaert CE, Croker BP, Harris CC, Seigler HF. In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody. Ann Surg. 1981 Nov;194(5):592–601.
Stuhlmiller, G. M., et al. “In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody.Ann Surg, vol. 194, no. 5, Nov. 1981, pp. 592–601. Pubmed, doi:10.1097/00000658-198111000-00007.
Stuhlmiller GM, Sullivan DC, Vervaert CE, Croker BP, Harris CC, Seigler HF. In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody. Ann Surg. 1981 Nov;194(5):592–601.

Published In

Ann Surg

DOI

ISSN

0003-4932

Publication Date

November 1981

Volume

194

Issue

5

Start / End Page

592 / 601

Location

United States

Related Subject Headings

  • Surgery
  • Radionuclide Imaging
  • Neoplasms, Experimental
  • Neoplasm Transplantation
  • Neoplasm Proteins
  • Mice, Nude
  • Mice
  • Melanoma-Specific Antigens
  • Melanoma
  • Macaca mulatta