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Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy.

Publication ,  Journal Article
Sostman, HD; Rockwell, S; Sylvia, AL; Madwed, D; Cofer, G; Charles, HC; Negro-Vilar, R; Moore, D
Published in: Magn Reson Med
August 1991

We studied tumor tissue oxygenation in the BA1112 rhabdomyosarcoma using micro-electrode pO2 measurements, optical spectrophotometry, analyses of cell survival after irradiation, and 31P magnetic resonance spectroscopy (MRS). Studies were carried out in WAG/Rij/Y rats breathing normoxic, hypoxic, and hyperoxic gas mixtures with and without iv administration of perfluorochemical. Significant changes in tissue oxygenation and metabolic status were found when pO2 values, optical measurements of hemoglobin saturation and cytochrome a, a3 reduction-oxidation, radiation cell survival determinations, and MRS measurements of phosphometabolite ratios were obtained in rats breathing different gas mixtures. Inhalation of 100% O2 caused increases in tumor pO2, hemoglobin saturation, cytochrome a, a3 oxidation, tumor radiosensitivity, and PCr/Pi, NTP/Pi, and PDE/Pi ratios. Such changes were augmented by pretreatment with iv perfluorochemicals. Inhalation of hypoxic gas mixtures resulted in reductions in the above parameters. These results indicate that tissue oxygenation can be manipulated reproducibly in the BA1112 rhabdomyosarcoma and suggest that 31P MRS can be used to monitor changes in tumor oxygenation in this model system.

Duke Scholars

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

August 1991

Volume

20

Issue

2

Start / End Page

253 / 267

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rhabdomyosarcoma
  • Rats
  • Radiation Tolerance
  • Oxygen Consumption
  • Nuclear Medicine & Medical Imaging
  • Microelectrodes
  • Magnetic Resonance Spectroscopy
  • Hydrocarbons, Brominated
  • Fluorocarbons
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sostman, H. D., Rockwell, S., Sylvia, A. L., Madwed, D., Cofer, G., Charles, H. C., … Moore, D. (1991). Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy. Magn Reson Med, 20(2), 253–267. https://doi.org/10.1002/mrm.1910200208
Sostman, H. D., S. Rockwell, A. L. Sylvia, D. Madwed, G. Cofer, H. C. Charles, R. Negro-Vilar, and D. Moore. “Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy.Magn Reson Med 20, no. 2 (August 1991): 253–67. https://doi.org/10.1002/mrm.1910200208.
Sostman HD, Rockwell S, Sylvia AL, Madwed D, Cofer G, Charles HC, et al. Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy. Magn Reson Med. 1991 Aug;20(2):253–67.
Sostman, H. D., et al. “Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy.Magn Reson Med, vol. 20, no. 2, Aug. 1991, pp. 253–67. Pubmed, doi:10.1002/mrm.1910200208.
Sostman HD, Rockwell S, Sylvia AL, Madwed D, Cofer G, Charles HC, Negro-Vilar R, Moore D. Evaluation of BA1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy. Magn Reson Med. 1991 Aug;20(2):253–267.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

August 1991

Volume

20

Issue

2

Start / End Page

253 / 267

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rhabdomyosarcoma
  • Rats
  • Radiation Tolerance
  • Oxygen Consumption
  • Nuclear Medicine & Medical Imaging
  • Microelectrodes
  • Magnetic Resonance Spectroscopy
  • Hydrocarbons, Brominated
  • Fluorocarbons