
On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.
Publication
, Journal Article
Arshavsky VYu; Antoch, MP; Philippov, PP
Published in: FEBS Lett
November 16, 1987
The rate of GTP hydrolysis in the active site of transducin and that of the release of the phosphate thus formed have been measured. The former step has been found to be a rate-limiting one. The rate constant for GTP hydrolysis is equal to 0.027 s-1 at 23 degrees C, and 0.07 s-1 at 37 degrees C. Besides, it has been shown that the rate of GTPase reaction on the transducin alpha-subunit does not depend on the concentration of a complex of transducin beta- and gamma-subunits or on the presence of cGMP phosphodiesterase and a 48 kDa protein from rod outer segments. According to the results, GTP hydrolysis on transducin proceeds too slowly to account for the rapid quenching of a phosphodiesterase cascade in rod outer segments.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
November 16, 1987
Volume
224
Issue
1
Start / End Page
19 / 22
Location
England
Related Subject Headings
- Vision, Ocular
- Transducin
- Rod Cell Outer Segment
- Rhodopsin
- Photoreceptor Cells
- Photic Stimulation
- Phosphorylation
- Phosphoric Monoester Hydrolases
- Membrane Proteins
- Guanosine Triphosphate
Citation
APA
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ICMJE
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Arshavsky VYu, Antoch, M. P., & Philippov, P. P. (1987). On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett, 224(1), 19–22. https://doi.org/10.1016/0014-5793(87)80414-3
Arshavsky VYu, M. P. Antoch, and P. P. Philippov. “On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.” FEBS Lett 224, no. 1 (November 16, 1987): 19–22. https://doi.org/10.1016/0014-5793(87)80414-3.
Arshavsky VYu, Antoch MP, Philippov PP. On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett. 1987 Nov 16;224(1):19–22.
Arshavsky VYu, et al. “On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.” FEBS Lett, vol. 224, no. 1, Nov. 1987, pp. 19–22. Pubmed, doi:10.1016/0014-5793(87)80414-3.
Arshavsky VYu, Antoch MP, Philippov PP. On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett. 1987 Nov 16;224(1):19–22.

Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
November 16, 1987
Volume
224
Issue
1
Start / End Page
19 / 22
Location
England
Related Subject Headings
- Vision, Ocular
- Transducin
- Rod Cell Outer Segment
- Rhodopsin
- Photoreceptor Cells
- Photic Stimulation
- Phosphorylation
- Phosphoric Monoester Hydrolases
- Membrane Proteins
- Guanosine Triphosphate