Scholars@Duke publication: On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.

On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.

Publication , Journal Article

Arshavsky VYu; Antoch, MP; Philippov, PP

Published in: FEBS Lett

November 16, 1987

The rate of GTP hydrolysis in the active site of transducin and that of the release of the phosphate thus formed have been measured. The former step has been found to be a rate-limiting one. The rate constant for GTP hydrolysis is equal to 0.027 s-1 at 23 degrees C, and 0.07 s-1 at 37 degrees C. Besides, it has been shown that the rate of GTPase reaction on the transducin alpha-subunit does not depend on the concentration of a complex of transducin beta- and gamma-subunits or on the presence of cGMP phosphodiesterase and a 48 kDa protein from rod outer segments. According to the results, GTP hydrolysis on transducin proceeds too slowly to account for the rapid quenching of a phosphodiesterase cascade in rod outer segments.

Duke Scholars

Author Vadim Y Arshavsky Ophthalmology, Vitreoretinal Diseases & Surgery

Published In

FEBS Lett

DOI

10.1016/0014-5793(87)80414-3

ISSN

0014-5793

Publication Date

November 16, 1987

Volume

224

Issue

Start / End Page

19 / 22

Location

England

Related Subject Headings

Vision, Ocular
Transducin
Rod Cell Outer Segment
Rhodopsin
Photoreceptor Cells
Photic Stimulation
Phosphorylation
Phosphoric Monoester Hydrolases
Membrane Proteins
Guanosine Triphosphate

Citation

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Arshavsky VYu, Antoch, M. P., & Philippov, P. P. (1987). On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett, 224(1), 19–22. https://doi.org/10.1016/0014-5793(87)80414-3

Arshavsky VYu, M. P. Antoch, and P. P. Philippov. “On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.” FEBS Lett 224, no. 1 (November 16, 1987): 19–22. https://doi.org/10.1016/0014-5793(87)80414-3.

Arshavsky VYu, Antoch MP, Philippov PP. On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett. 1987 Nov 16;224(1):19–22.

Arshavsky VYu, et al. “On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.” FEBS Lett, vol. 224, no. 1, Nov. 1987, pp. 19–22. Pubmed, doi:10.1016/0014-5793(87)80414-3.

Arshavsky VYu, Antoch MP, Philippov PP. On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. FEBS Lett. 1987 Nov 16;224(1):19–22.