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Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors.

Publication ,  Journal Article
Strissel, KJ; Lishko, PV; Trieu, LH; Kennedy, MJ; Hurley, JB; Arshavsky, VY
Published in: J Biol Chem
August 12, 2005

Photoreceptor cells have a remarkable capacity to adapt the sensitivity and speed of their responses to ever changing conditions of ambient illumination. Recent studies have revealed that a major contributor to this adaptation is the phenomenon of light-driven translocation of key signaling proteins into and out of the photoreceptor outer segment, the cellular compartment where phototransduction takes place. So far, only two such proteins, transducin and arrestin, have been established to be involved in this mechanism. To investigate the extent of this phenomenon we examined additional photoreceptor proteins that might undergo light-driven translocation, focusing on three Ca(2+)-binding proteins, recoverin and guanylate cyclase activating proteins 1 (GCAP1) and GCAP2. The changes in the subcellular distribution of each protein were assessed quantitatively using a recently developed technique combining serial tangential sectioning of mouse retinas with Western blot analysis of the proteins in the individual sections. Our major finding is that light causes a significant reduction of recoverin in rod outer segments, accompanied by its redistribution toward rod synaptic terminals. In both cases the majority of recoverin was found in rod inner segments, with approximately 12% present in the outer segments in the dark and less than 2% remaining in that compartment in the light. We suggest that recoverin translocation is adaptive because it may reduce the inhibitory constraint that recoverin imposes on rhodopsin kinase, an enzyme responsible for quenching the photo-excited rhodopsin during the photoresponse. To the contrary, no translocation of rhodopsin kinase itself or either GCAP was identified.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

August 12, 2005

Volume

280

Issue

32

Start / End Page

29250 / 29255

Location

United States

Related Subject Headings

  • beta-Cyclodextrins
  • Signal Transduction
  • Rhodopsin
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Recoverin
  • RNA, Messenger
  • Protein Transport
  • Protein Kinase Inhibitors
  • Mice, Inbred C57BL
 

Citation

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Strissel, K. J., Lishko, P. V., Trieu, L. H., Kennedy, M. J., Hurley, J. B., & Arshavsky, V. Y. (2005). Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. J Biol Chem, 280(32), 29250–29255. https://doi.org/10.1074/jbc.M501789200
Strissel, Katherine J., Polina V. Lishko, Lynn H. Trieu, Matthew J. Kennedy, James B. Hurley, and Vadim Y. Arshavsky. “Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors.J Biol Chem 280, no. 32 (August 12, 2005): 29250–55. https://doi.org/10.1074/jbc.M501789200.
Strissel KJ, Lishko PV, Trieu LH, Kennedy MJ, Hurley JB, Arshavsky VY. Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. J Biol Chem. 2005 Aug 12;280(32):29250–5.
Strissel, Katherine J., et al. “Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors.J Biol Chem, vol. 280, no. 32, Aug. 2005, pp. 29250–55. Pubmed, doi:10.1074/jbc.M501789200.
Strissel KJ, Lishko PV, Trieu LH, Kennedy MJ, Hurley JB, Arshavsky VY. Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. J Biol Chem. 2005 Aug 12;280(32):29250–29255.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

August 12, 2005

Volume

280

Issue

32

Start / End Page

29250 / 29255

Location

United States

Related Subject Headings

  • beta-Cyclodextrins
  • Signal Transduction
  • Rhodopsin
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Recoverin
  • RNA, Messenger
  • Protein Transport
  • Protein Kinase Inhibitors
  • Mice, Inbred C57BL