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Sustained stress response after oxidative stress in trabecular meshwork cells.

Publication ,  Journal Article
Li, G; Luna, C; Liton, PB; Navarro, I; Epstein, DL; Gonzalez, P
Published in: Mol Vis
December 17, 2007

PURPOSE: To investigate the mechanisms by which chronic oxidative stress may lead to a sustained stress response similar to that previously observed in the trabecular meshwork (TM) of glaucoma donors. METHODS: Porcine TM cells were treated with 200 microM H2O2 twice a day for four days and were allowed to recover for three additional days. After the treatment, TM cells were analyzed for generation of intracellular reactive oxygen species (iROS), mitochondrial potential, activation of NF-kappaB, and the expression of inflammatory markers IL-1alpha, IL-6, IL-8, and ELAM-1. Potential sources of iROS were evaluated using inhibitors for nitric oxide, nitric oxide synthetase, cyclooxygenase, xanthine oxidase, NADPH oxidase, mitochondrial ROS, and PKC. The role of NF-kappaB activation in the induction of inflammatory markers was evaluated using the inhibitors Lactacystin and BAY11-7082. RESULTS: Chronic oxidative stress simulated by H2O2 exposure of porcine TM cells resulted in the sustained production of iROS by the mitochondria. Inhibition of mitochondrial iROS had a significant inhibitory effect on the activation of NF-kappaB and the induction of IL-1alpha, IL-6, IL-8, and ELAM-1 triggered by chronic oxidative stress. Inhibition of NF-kappaB partially prevented the induction of IL-1alpha, IL-8, and ELAM-1, but not IL-6. CONCLUSIONS: Chronic oxidative stress in TM cells induced iROS production in mitochondria. This increase in iROS may contribute to the pathogenesis of the TM in glaucoma by inducing the expression of inflammatory mediators previously observed in glaucoma donors as well as the levels of oxidative damage in the tissue.

Duke Scholars

Published In

Mol Vis

EISSN

1090-0535

Publication Date

December 17, 2007

Volume

13

Start / End Page

2282 / 2288

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Time Factors
  • Signal Transduction
  • Reactive Oxygen Species
  • Oxidative Stress
  • Ophthalmology & Optometry
  • NF-kappa B
  • Mitochondria
  • Membrane Potential, Mitochondrial
  • Interleukins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, G., Luna, C., Liton, P. B., Navarro, I., Epstein, D. L., & Gonzalez, P. (2007). Sustained stress response after oxidative stress in trabecular meshwork cells. Mol Vis, 13, 2282–2288.
Li, Guorong, Coralia Luna, Paloma B. Liton, Iris Navarro, David L. Epstein, and Pedro Gonzalez. “Sustained stress response after oxidative stress in trabecular meshwork cells.Mol Vis 13 (December 17, 2007): 2282–88.
Li G, Luna C, Liton PB, Navarro I, Epstein DL, Gonzalez P. Sustained stress response after oxidative stress in trabecular meshwork cells. Mol Vis. 2007 Dec 17;13:2282–8.
Li, Guorong, et al. “Sustained stress response after oxidative stress in trabecular meshwork cells.Mol Vis, vol. 13, Dec. 2007, pp. 2282–88.
Li G, Luna C, Liton PB, Navarro I, Epstein DL, Gonzalez P. Sustained stress response after oxidative stress in trabecular meshwork cells. Mol Vis. 2007 Dec 17;13:2282–2288.

Published In

Mol Vis

EISSN

1090-0535

Publication Date

December 17, 2007

Volume

13

Start / End Page

2282 / 2288

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Time Factors
  • Signal Transduction
  • Reactive Oxygen Species
  • Oxidative Stress
  • Ophthalmology & Optometry
  • NF-kappa B
  • Mitochondria
  • Membrane Potential, Mitochondrial
  • Interleukins