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Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.

Publication ,  Journal Article
Lobanova, ES; Finkelstein, S; Herrmann, R; Chen, Y-M; Kessler, C; Michaud, NA; Trieu, LH; Strissel, KJ; Burns, ME; Arshavsky, VY
Published in: J Neurosci
March 26, 2008

Transducin is a prototypic heterotrimeric G-protein mediating visual signaling in vertebrate photoreceptor cells. Despite its central role in phototransduction, little is known about the mechanisms that regulate its expression and maintain approximately stoichiometric levels of the alpha- and betagamma-subunits. Here we demonstrate that the knock-out of transducin gamma-subunit leads to a major downregulation of both alpha- and beta-subunit proteins, despite nearly normal levels of the corresponding transcripts, and fairly rapid photoreceptor degeneration. Significant fractions of the remaining alpha- and beta-subunits were mislocalized from the light-sensitive outer segment compartment of the rod. Yet, the tiny amount of the alpha-subunit present in the outer segments of knock-out rods was sufficient to support light signaling, although with a markedly reduced sensitivity. These data indicate that the gamma-subunit controls the expression level of the entire transducin heterotrimer and that heterotrimer formation is essential for normal transducin localization. They further suggest that the production of transducin beta-subunit without its constitutive gamma-subunit partner sufficiently stresses the cellular biosynthetic and/or chaperone machinery to induce cell death.

Duke Scholars

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

March 26, 2008

Volume

28

Issue

13

Start / End Page

3510 / 3520

Location

United States

Related Subject Headings

  • Transducin
  • Rod Cell Outer Segment
  • Retinal Rod Photoreceptor Cells
  • Phosphoproteins
  • Patch-Clamp Techniques
  • Neurology & Neurosurgery
  • Microscopy, Electron, Transmission
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lobanova, E. S., Finkelstein, S., Herrmann, R., Chen, Y.-M., Kessler, C., Michaud, N. A., … Arshavsky, V. Y. (2008). Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability. J Neurosci, 28(13), 3510–3520. https://doi.org/10.1523/JNEUROSCI.0338-08.2008
Lobanova, Ekaterina S., Stella Finkelstein, Rolf Herrmann, Yen-Ming Chen, Christopher Kessler, Norman A. Michaud, Lynn H. Trieu, Katherine J. Strissel, Marie E. Burns, and Vadim Y. Arshavsky. “Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.J Neurosci 28, no. 13 (March 26, 2008): 3510–20. https://doi.org/10.1523/JNEUROSCI.0338-08.2008.
Lobanova ES, Finkelstein S, Herrmann R, Chen Y-M, Kessler C, Michaud NA, et al. Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability. J Neurosci. 2008 Mar 26;28(13):3510–20.
Lobanova, Ekaterina S., et al. “Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.J Neurosci, vol. 28, no. 13, Mar. 2008, pp. 3510–20. Pubmed, doi:10.1523/JNEUROSCI.0338-08.2008.
Lobanova ES, Finkelstein S, Herrmann R, Chen Y-M, Kessler C, Michaud NA, Trieu LH, Strissel KJ, Burns ME, Arshavsky VY. Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability. J Neurosci. 2008 Mar 26;28(13):3510–3520.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

March 26, 2008

Volume

28

Issue

13

Start / End Page

3510 / 3520

Location

United States

Related Subject Headings

  • Transducin
  • Rod Cell Outer Segment
  • Retinal Rod Photoreceptor Cells
  • Phosphoproteins
  • Patch-Clamp Techniques
  • Neurology & Neurosurgery
  • Microscopy, Electron, Transmission
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice