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RGS expression rate-limits recovery of rod photoresponses.

Publication ,  Journal Article
Krispel, CM; Chen, D; Melling, N; Chen, Y-J; Martemyanov, KA; Quillinan, N; Arshavsky, VY; Wensel, TG; Chen, C-K; Burns, ME
Published in: Neuron
August 17, 2006

Signaling through G protein-coupled receptors (GPCRs) underlies many cellular processes, yet it is not known which molecules determine the duration of signaling in intact cells. Two candidates are G protein-coupled receptor kinases (GRKs) and Regulators of G protein signaling (RGSs), deactivation enzymes for GPCRs and G proteins, respectively. Here we investigate whether GRK or RGS governs the overall rate of recovery of the light response in mammalian rod photoreceptors, a model system for studying GPCR signaling. We show that overexpression of rhodopsin kinase (GRK1) increases phosphorylation of the GPCR rhodopsin but has no effect on photoresponse recovery. In contrast, overexpression of the photoreceptor RGS complex (RGS9-1.Gbeta5L.R9AP) dramatically accelerates response recovery. Our results show that G protein deactivation is normally at least 2.5 times slower than rhodopsin deactivation, resolving a long-standing controversy concerning the mechanism underlying the recovery of rod visual transduction.

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Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

August 17, 2006

Volume

51

Issue

4

Start / End Page

409 / 416

Location

United States

Related Subject Headings

  • Vision, Ocular
  • Time Factors
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Recovery of Function
  • RGS Proteins
  • Photic Stimulation
  • Phosphorylation
  • Neurology & Neurosurgery
  • Mice, Transgenic
 

Citation

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Krispel, C. M., Chen, D., Melling, N., Chen, Y.-J., Martemyanov, K. A., Quillinan, N., … Burns, M. E. (2006). RGS expression rate-limits recovery of rod photoresponses. Neuron, 51(4), 409–416. https://doi.org/10.1016/j.neuron.2006.07.010
Krispel, Claudia M., Desheng Chen, Nathan Melling, Yu-Jiun Chen, Kirill A. Martemyanov, Nidia Quillinan, Vadim Y. Arshavsky, Theodore G. Wensel, Ching-Kang Chen, and Marie E. Burns. “RGS expression rate-limits recovery of rod photoresponses.Neuron 51, no. 4 (August 17, 2006): 409–16. https://doi.org/10.1016/j.neuron.2006.07.010.
Krispel CM, Chen D, Melling N, Chen Y-J, Martemyanov KA, Quillinan N, et al. RGS expression rate-limits recovery of rod photoresponses. Neuron. 2006 Aug 17;51(4):409–16.
Krispel, Claudia M., et al. “RGS expression rate-limits recovery of rod photoresponses.Neuron, vol. 51, no. 4, Aug. 2006, pp. 409–16. Pubmed, doi:10.1016/j.neuron.2006.07.010.
Krispel CM, Chen D, Melling N, Chen Y-J, Martemyanov KA, Quillinan N, Arshavsky VY, Wensel TG, Chen C-K, Burns ME. RGS expression rate-limits recovery of rod photoresponses. Neuron. 2006 Aug 17;51(4):409–416.
Journal cover image

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

August 17, 2006

Volume

51

Issue

4

Start / End Page

409 / 416

Location

United States

Related Subject Headings

  • Vision, Ocular
  • Time Factors
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Recovery of Function
  • RGS Proteins
  • Photic Stimulation
  • Phosphorylation
  • Neurology & Neurosurgery
  • Mice, Transgenic