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Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation.

Publication ,  Journal Article
Rajala, A; Daly, RJ; Tanito, M; Allen, DT; Holt, LJ; Lobanova, ES; Arshavsky, VY; Rajala, RVS
Published in: Biochemistry
June 23, 2009

Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclear layer, and synapse in dark-adapted rods, whereas in the light-adapted rods, Grb14 redistributed throughout the entire cell, including the outer segment. The translocation of Grb14 requires photoactivation of rhodopsin, but not signaling through the phototransduction cascade, and is not based on direct Grb14-rhodopsin interactions. We previously hypothesized that Grb14 protects light-dependent insulin receptor (IR) activation in rod photoreceptors against dephosphorylation by protein tyrosine phosphatase 1B. Consistent with this hypothesis, we failed to observe light-dependent IR activation in Grb14(-/-) mouse retinas. Our studies suggest that Grb14 translocates to photoreceptor outer segments after photobleaching of rhodopsin and protects IR phosphorylation in rod photoreceptor cells. These results demonstrate that Grb14 can undergo subcellular redistribution upon illumination and suggest that rhodopsin photoexcitation may trigger signaling events alternative to the classical transducin activation.

Duke Scholars

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

June 23, 2009

Volume

48

Issue

24

Start / End Page

5563 / 5572

Location

United States

Related Subject Headings

  • cis-trans-Isomerases
  • Transducin
  • Signal Transduction
  • Rod Cell Outer Segment
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Receptor, Insulin
  • Rats, Inbred Strains
  • Rats
  • Proteins
 

Citation

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Rajala, A., Daly, R. J., Tanito, M., Allen, D. T., Holt, L. J., Lobanova, E. S., … Rajala, R. V. S. (2009). Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation. Biochemistry, 48(24), 5563–5572. https://doi.org/10.1021/bi9000062
Rajala, Ammaji, Roger J. Daly, Masaki Tanito, Dustin T. Allen, Lowenna J. Holt, Ekaterina S. Lobanova, Vadim Y. Arshavsky, and Raju V. S. Rajala. “Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation.Biochemistry 48, no. 24 (June 23, 2009): 5563–72. https://doi.org/10.1021/bi9000062.
Rajala, Ammaji, et al. “Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation.Biochemistry, vol. 48, no. 24, June 2009, pp. 5563–72. Pubmed, doi:10.1021/bi9000062.
Rajala A, Daly RJ, Tanito M, Allen DT, Holt LJ, Lobanova ES, Arshavsky VY, Rajala RVS. Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation. Biochemistry. 2009 Jun 23;48(24):5563–5572.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

June 23, 2009

Volume

48

Issue

24

Start / End Page

5563 / 5572

Location

United States

Related Subject Headings

  • cis-trans-Isomerases
  • Transducin
  • Signal Transduction
  • Rod Cell Outer Segment
  • Retinal Rod Photoreceptor Cells
  • Retina
  • Receptor, Insulin
  • Rats, Inbred Strains
  • Rats
  • Proteins