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Tomographic digital subtraction angiography for lung perfusion estimation in rodents.

Publication ,  Journal Article
Badea, CT; Hedlund, LW; De Lin, M; Mackel, JSB; Samei, E; Johnson, GA
Published in: Med Phys
May 2007

In vivo measurements of perfusion present a challenge to existing small animal imaging techniques such as magnetic resonance microscopy, micro computed tomography, micro positron emission tomography, and microSPECT, due to combined requirements for high spatial and temporal resolution. We demonstrate the use of tomographic digital subtraction angiography (TDSA) for estimation of perfusion in small animals. TDSA augments conventional digital subtraction angiography (DSA) by providing three-dimensional spatial information using tomosynthesis algorithms. TDSA is based on the novel paradigm that the same time density curves can be reproduced in a number of consecutive injections of microL volumes of contrast at a series of different angles of rotation. The capabilities of TDSA are established in studies on lung perfusion in rats. Using an imaging system developed in-house, we acquired data for four-dimensional (4D) imaging with temporal resolution of 140 ms, in-plane spatial resolution of 100 microm, and slice thickness on the order of millimeters. Based on a structured experimental approach, we optimized TDSA imaging providing a good trade-off between slice thickness, the number of injections, contrast to noise, and immunity to artifacts. Both DSA and TDSA images were used to create parametric maps of perfusion. TDSA imaging has potential application in a number of areas where functional perfusion measurements in 4D can provide valuable insight into animal models of disease and response to therapeutics.

Duke Scholars

Published In

Med Phys

DOI

ISSN

0094-2405

Publication Date

May 2007

Volume

34

Issue

5

Start / End Page

1546 / 1555

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Rats, Inbred F344
  • Rats
  • Radiographic Image Enhancement
  • Perfusion
  • Nuclear Medicine & Medical Imaging
  • Magnetic Resonance Angiography
  • Lung
  • Female
  • Animals
 

Citation

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MLA
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Badea, C. T., Hedlund, L. W., De Lin, M., Mackel, J. S. B., Samei, E., & Johnson, G. A. (2007). Tomographic digital subtraction angiography for lung perfusion estimation in rodents. Med Phys, 34(5), 1546–1555. https://doi.org/10.1118/1.2717384
Badea, Cristian T., Laurence W. Hedlund, Ming De Lin, Julie S Boslego Mackel, Ehsan Samei, and G Allan Johnson. “Tomographic digital subtraction angiography for lung perfusion estimation in rodents.Med Phys 34, no. 5 (May 2007): 1546–55. https://doi.org/10.1118/1.2717384.
Badea CT, Hedlund LW, De Lin M, Mackel JSB, Samei E, Johnson GA. Tomographic digital subtraction angiography for lung perfusion estimation in rodents. Med Phys. 2007 May;34(5):1546–55.
Badea, Cristian T., et al. “Tomographic digital subtraction angiography for lung perfusion estimation in rodents.Med Phys, vol. 34, no. 5, May 2007, pp. 1546–55. Pubmed, doi:10.1118/1.2717384.
Badea CT, Hedlund LW, De Lin M, Mackel JSB, Samei E, Johnson GA. Tomographic digital subtraction angiography for lung perfusion estimation in rodents. Med Phys. 2007 May;34(5):1546–1555.

Published In

Med Phys

DOI

ISSN

0094-2405

Publication Date

May 2007

Volume

34

Issue

5

Start / End Page

1546 / 1555

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Rats, Inbred F344
  • Rats
  • Radiographic Image Enhancement
  • Perfusion
  • Nuclear Medicine & Medical Imaging
  • Magnetic Resonance Angiography
  • Lung
  • Female
  • Animals