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In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe.

Publication ,  Journal Article
Cleveland, ZI; Möller, HE; Hedlund, LW; Nouls, JC; Freeman, MS; Qi, Y; Driehuys, B
Published in: PLoS One
2012

BACKGROUND: Hyperpolarized (HP) (129)Xe magnetic resonance imaging (MRI) permits high resolution, regional visualization of pulmonary ventilation. Additionally, its reasonably high solubility (>10%) and large chemical shift range (>200 ppm) in tissues allow HP (129)Xe to serve as a regional probe of pulmonary perfusion and gas transport, when introduced directly into the vasculature. In earlier work, vascular delivery was accomplished in rats by first dissolving HP (129)Xe in a biologically compatible carrier solution, injecting the solution into the vasculature, and then detecting HP (129)Xe as it emerged into the alveolar airspaces. Although easily implemented, this approach was constrained by the tolerable injection volume and the duration of the HP (129)Xe signal. METHODS AND PRINCIPAL FINDINGS: Here, we overcome the volume and temporal constraints imposed by injection, by using hydrophobic, microporous, gas-exchange membranes to directly and continuously infuse (129)Xe into the arterial blood of live rats with an extracorporeal (EC) circuit. The resulting gas-phase (129)Xe signal is sufficient to generate diffusive gas exchange- and pulmonary perfusion-dependent, 3D MR images with a nominal resolution of 2×2×2 mm(3). We also show that the (129)Xe signal dynamics during EC infusion are well described by an analytical model that incorporates both mass transport into the blood and longitudinal relaxation. CONCLUSIONS: Extracorporeal infusion of HP (129)Xe enables rapid, 3D MR imaging of rat lungs and, when combined with ventilation imaging, will permit spatially resolved studies of the ventilation-perfusion ratio in small animals. Moreover, EC infusion should allow (129)Xe to be delivered elsewhere in the body and make possible functional and molecular imaging approaches that are currently not feasible using inhaled HP (129)Xe.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2012

Volume

7

Issue

2

Start / End Page

e31306

Location

United States

Related Subject Headings

  • Xenon Isotopes
  • Xenon
  • Signal Processing, Computer-Assisted
  • Respiration
  • Rats, Sprague-Dawley
  • Rats
  • Pulmonary Gas Exchange
  • Perfusion
  • Models, Biological
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
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Cleveland, Z. I., Möller, H. E., Hedlund, L. W., Nouls, J. C., Freeman, M. S., Qi, Y., & Driehuys, B. (2012). In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe. PLoS One, 7(2), e31306. https://doi.org/10.1371/journal.pone.0031306
Cleveland, Zackary I., Harald E. Möller, Laurence W. Hedlund, John C. Nouls, Matthew S. Freeman, Yi Qi, and Bastiaan Driehuys. “In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe.PLoS One 7, no. 2 (2012): e31306. https://doi.org/10.1371/journal.pone.0031306.
Cleveland ZI, Möller HE, Hedlund LW, Nouls JC, Freeman MS, Qi Y, et al. In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe. PLoS One. 2012;7(2):e31306.
Cleveland, Zackary I., et al. “In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe.PLoS One, vol. 7, no. 2, 2012, p. e31306. Pubmed, doi:10.1371/journal.pone.0031306.
Cleveland ZI, Möller HE, Hedlund LW, Nouls JC, Freeman MS, Qi Y, Driehuys B. In vivo MR imaging of pulmonary perfusion and gas exchange in rats via continuous extracorporeal infusion of hyperpolarized 129Xe. PLoS One. 2012;7(2):e31306.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2012

Volume

7

Issue

2

Start / End Page

e31306

Location

United States

Related Subject Headings

  • Xenon Isotopes
  • Xenon
  • Signal Processing, Computer-Assisted
  • Respiration
  • Rats, Sprague-Dawley
  • Rats
  • Pulmonary Gas Exchange
  • Perfusion
  • Models, Biological
  • Male