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Induction of anti-myelin antibodies in EAE and their possible role in demyelination.

Publication ,  Journal Article
Sadler, RH; Sommer, MA; Forno, LS; Smith, ME
Published in: Journal of neuroscience research
December 1991

Experimental allergic encephalomyelitis is characterized by invasion of lymphocytes and macrophages into the central nervous system resulting in inflammation, edema, and demyelination. Sera from Lewis rats from 7-95 days after immunization with purified guinea pig CNS myelin were examined with respect to their ability to opsonize myelin. This was correlated with the appearance of antibody components and the relative amounts of antibody to myelin basic protein (MBP) and proteolipid protein (PLP). Sera from rats 10-95 days after immunization preincubated with purified myelin induced phagocytosis of myelin by cultured macrophages with the resulting production of cholesterol ester. This opsonization activity as measured by the percentage of cholesterol esterified reached a peak at 26-27 days after immunization but remained significantly elevated up to 95 days post-immunization compared to the activity of serum from the Freund's adjuvant-injected controls. Immunoblots of the sera revealed a gradual increase in antibody activity against myelin components. ELISA assays for MBP and PLP antibody showed a similar pattern. Antibody to galactocerebroside (GC) was not detected by immunostains nor by the ELISA assay. Areas of demyelination were observed histologically by luxol-fast blue stained spinal cords up to 60 days post-immunization. These results indicate that antibodies to myelin protein when given access to myelin through or within the blood brain barrier could initiate or enhance the phagocytic response by peripheral or resident macrophages.

Duke Scholars

Published In

Journal of neuroscience research

DOI

EISSN

1097-4547

ISSN

0360-4012

Publication Date

December 1991

Volume

30

Issue

4

Start / End Page

616 / 624

Related Subject Headings

  • Rats, Inbred Lew
  • Rats
  • Phagocytosis
  • Neurology & Neurosurgery
  • Myelin Sheath
  • Myelin Proteins
  • Male
  • Enzyme-Linked Immunosorbent Assay
  • Encephalomyelitis, Autoimmune, Experimental
  • Demyelinating Diseases
 

Citation

APA
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ICMJE
MLA
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Sadler, R. H., Sommer, M. A., Forno, L. S., & Smith, M. E. (1991). Induction of anti-myelin antibodies in EAE and their possible role in demyelination. Journal of Neuroscience Research, 30(4), 616–624. https://doi.org/10.1002/jnr.490300404
Sadler, R. H., M. A. Sommer, L. S. Forno, and M. E. Smith. “Induction of anti-myelin antibodies in EAE and their possible role in demyelination.Journal of Neuroscience Research 30, no. 4 (December 1991): 616–24. https://doi.org/10.1002/jnr.490300404.
Sadler RH, Sommer MA, Forno LS, Smith ME. Induction of anti-myelin antibodies in EAE and their possible role in demyelination. Journal of neuroscience research. 1991 Dec;30(4):616–24.
Sadler, R. H., et al. “Induction of anti-myelin antibodies in EAE and their possible role in demyelination.Journal of Neuroscience Research, vol. 30, no. 4, Dec. 1991, pp. 616–24. Epmc, doi:10.1002/jnr.490300404.
Sadler RH, Sommer MA, Forno LS, Smith ME. Induction of anti-myelin antibodies in EAE and their possible role in demyelination. Journal of neuroscience research. 1991 Dec;30(4):616–624.
Journal cover image

Published In

Journal of neuroscience research

DOI

EISSN

1097-4547

ISSN

0360-4012

Publication Date

December 1991

Volume

30

Issue

4

Start / End Page

616 / 624

Related Subject Headings

  • Rats, Inbred Lew
  • Rats
  • Phagocytosis
  • Neurology & Neurosurgery
  • Myelin Sheath
  • Myelin Proteins
  • Male
  • Enzyme-Linked Immunosorbent Assay
  • Encephalomyelitis, Autoimmune, Experimental
  • Demyelinating Diseases