Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
Publication
, Journal Article
Tolun, AA; Zhang, H; Il'yasova, D; Sztáray, J; Young, SP; Millington, DS
Published in: Anal Biochem
July 15, 2010
Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.
Duke Scholars
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
July 15, 2010
Volume
402
Issue
2
Start / End Page
191 / 193
Location
United States
Related Subject Headings
- Young Adult
- Tandem Mass Spectrometry
- Middle Aged
- Male
- Limit of Detection
- Humans
- Female
- Chromatography, High Pressure Liquid
- Child, Preschool
- Child
Citation
APA
Chicago
ICMJE
MLA
NLM
Tolun, A. A., Zhang, H., Il’yasova, D., Sztáray, J., Young, S. P., & Millington, D. S. (2010). Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry. Anal Biochem, 402(2), 191–193. https://doi.org/10.1016/j.ab.2010.03.033
Tolun, Adviye A., Haoyue Zhang, Dora Il’yasova, Judit Sztáray, Sarah P. Young, and David S. Millington. “Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.” Anal Biochem 402, no. 2 (July 15, 2010): 191–93. https://doi.org/10.1016/j.ab.2010.03.033.
Tolun AA, Zhang H, Il’yasova D, Sztáray J, Young SP, Millington DS. Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry. Anal Biochem. 2010 Jul 15;402(2):191–3.
Tolun, Adviye A., et al. “Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.” Anal Biochem, vol. 402, no. 2, July 2010, pp. 191–93. Pubmed, doi:10.1016/j.ab.2010.03.033.
Tolun AA, Zhang H, Il’yasova D, Sztáray J, Young SP, Millington DS. Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry. Anal Biochem. 2010 Jul 15;402(2):191–193.
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
July 15, 2010
Volume
402
Issue
2
Start / End Page
191 / 193
Location
United States
Related Subject Headings
- Young Adult
- Tandem Mass Spectrometry
- Middle Aged
- Male
- Limit of Detection
- Humans
- Female
- Chromatography, High Pressure Liquid
- Child, Preschool
- Child