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Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937.

Publication ,  Journal Article
Gray, TK; Millington, DS; Maltby, DA; Williams, ME; Cohen, MS; Dodd, RC
Published in: Proc Natl Acad Sci U S A
December 1985

Phagocytic cells, including normal human blood neutrophils and monocytes, metabolize 25-hydroxyvitamin D3 in vitro to more polar metabolites. Cells of the human monoblastic cell line U937 produced three metabolites when incubated with 25-hydroxyvitamin D3. One of these metabolites, previously designated peak III, has its maximal absorbance at 310 nm. Mass spectral analysis of the trimethylsilylated derivatives of peak III revealed a spectral pattern very similar to that published for the trimethylsilylated derivatives of 19-nor-10-keto-25-hydroxyvitamin D3. The biosynthetic 19-nor-10-keto-25-hydroxyvitamin D3 was active in the induction of differentiation of U937 cells.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1985

Volume

82

Issue

23

Start / End Page

8218 / 8221

Location

United States

Related Subject Headings

  • Spectrophotometry, Ultraviolet
  • Phagocytes
  • Monocytes
  • Mass Spectrometry
  • Lymphokines
  • Cell Line
  • Cell Division
  • Cell Differentiation
  • Calcifediol
 

Citation

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MLA
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Gray, T. K., Millington, D. S., Maltby, D. A., Williams, M. E., Cohen, M. S., & Dodd, R. C. (1985). Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937. Proc Natl Acad Sci U S A, 82(23), 8218–8221. https://doi.org/10.1073/pnas.82.23.8218
Gray, T. K., D. S. Millington, D. A. Maltby, M. E. Williams, M. S. Cohen, and R. C. Dodd. “Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937.Proc Natl Acad Sci U S A 82, no. 23 (December 1985): 8218–21. https://doi.org/10.1073/pnas.82.23.8218.
Gray TK, Millington DS, Maltby DA, Williams ME, Cohen MS, Dodd RC. Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8218–21.
Gray, T. K., et al. “Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937.Proc Natl Acad Sci U S A, vol. 82, no. 23, Dec. 1985, pp. 8218–21. Pubmed, doi:10.1073/pnas.82.23.8218.
Gray TK, Millington DS, Maltby DA, Williams ME, Cohen MS, Dodd RC. Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8218–8221.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1985

Volume

82

Issue

23

Start / End Page

8218 / 8221

Location

United States

Related Subject Headings

  • Spectrophotometry, Ultraviolet
  • Phagocytes
  • Monocytes
  • Mass Spectrometry
  • Lymphokines
  • Cell Line
  • Cell Division
  • Cell Differentiation
  • Calcifediol