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High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples.

Publication ,  Journal Article
Leach, RE; Jessmon, P; Coutifaris, C; Kruger, M; Myers, ER; Ali-Fehmi, R; Carson, SA; Legro, RS; Schlaff, WD; Carr, BR; Steinkampf, MP ...
Published in: Hum Reprod
March 2012

BACKGROUND: Although histological dating of endometrial biopsies provides little help for prediction or diagnosis of infertility, analysis of individual endometrial proteins, proteomic profiling and transcriptome analysis have suggested several biomarkers with altered expression arising from intrinsic abnormalities, inadequate stimulation by or in response to gonadal steroids or altered function due to systemic disorders. The objective of this study was to delineate the developmental dynamics of potentially important proteins in the secretory phase of the menstrual cycle, utilizing a collection of endometrial biopsies from women of fertile (n = 89) and infertile (n = 89) couples. METHODS AND RESULTS: Progesterone receptor-B (PGR-B), leukemia inhibitory factor, glycodelin/progestagen-associated endometrial protein (PAEP), homeobox A10, heparin-binding EGF-like growth factor, calcitonin and chemokine ligand 14 (CXCL14) were measured using a high-throughput, quantitative immunohistochemical method. Significant cyclic and tissue-specific regulation was documented for each protein, as well as their dysregulation in women of infertile couples. Infertile patients demonstrated a delay early in the secretory phase in the decline of PGR-B (P < 0.05) and premature mid-secretory increases in PAEP (P < 0.05) and CXCL14 (P < 0.05), suggesting that the implantation interval could be closing early. Correlation analysis identified potential interactions among certain proteins that were disrupted by infertility. CONCLUSIONS: This approach overcomes the limitations of a small sample number. Protein expression and localization provided important insights into the potential roles of these proteins in normal and pathological development of the endometrium that is not attainable from transcriptome analysis, establishing a basis for biomarker, diagnostic and targeted drug development for women with infertility.

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Published In

Hum Reprod

DOI

EISSN

1460-2350

Publication Date

March 2012

Volume

27

Issue

3

Start / End Page

814 / 828

Location

England

Related Subject Headings

  • Receptors, Progesterone
  • Pregnancy Proteins
  • Obstetrics & Reproductive Medicine
  • Male
  • Leukemia Inhibitory Factor
  • Intercellular Signaling Peptides and Proteins
  • Infertility, Female
  • Immunohistochemistry
  • Humans
  • Heparin-binding EGF-like Growth Factor
 

Citation

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MLA
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Leach, R. E., Jessmon, P., Coutifaris, C., Kruger, M., Myers, E. R., Ali-Fehmi, R., … Reproductive Medicine Network, . (2012). High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod, 27(3), 814–828. https://doi.org/10.1093/humrep/der436
Leach, Richard E., Philip Jessmon, Christos Coutifaris, Michael Kruger, Evan R. Myers, Rouba Ali-Fehmi, Sandra A. Carson, et al. “High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples.Hum Reprod 27, no. 3 (March 2012): 814–28. https://doi.org/10.1093/humrep/der436.
Leach RE, Jessmon P, Coutifaris C, Kruger M, Myers ER, Ali-Fehmi R, et al. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod. 2012 Mar;27(3):814–28.
Leach, Richard E., et al. “High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples.Hum Reprod, vol. 27, no. 3, Mar. 2012, pp. 814–28. Pubmed, doi:10.1093/humrep/der436.
Leach RE, Jessmon P, Coutifaris C, Kruger M, Myers ER, Ali-Fehmi R, Carson SA, Legro RS, Schlaff WD, Carr BR, Steinkampf MP, Silva S, Leppert PC, Giudice L, Diamond MP, Armant DR, Reproductive Medicine Network. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod. 2012 Mar;27(3):814–828.
Journal cover image

Published In

Hum Reprod

DOI

EISSN

1460-2350

Publication Date

March 2012

Volume

27

Issue

3

Start / End Page

814 / 828

Location

England

Related Subject Headings

  • Receptors, Progesterone
  • Pregnancy Proteins
  • Obstetrics & Reproductive Medicine
  • Male
  • Leukemia Inhibitory Factor
  • Intercellular Signaling Peptides and Proteins
  • Infertility, Female
  • Immunohistochemistry
  • Humans
  • Heparin-binding EGF-like Growth Factor