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Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors.

Publication ,  Journal Article
Polstein, LR; Gersbach, CA
Published in: Journal of the American Chemical Society
October 2012

Advanced gene regulatory systems are necessary for scientific research, synthetic biology, and gene-based medicine. An ideal system would allow facile spatiotemporal manipulation of gene expression within a cell population that is tunable, reversible, repeatable, and can be targeted to diverse DNA sequences. To meet these criteria, a gene regulation system was engineered that combines light-sensitive proteins and programmable zinc finger transcription factors. This system, light-inducible transcription using engineered zinc finger proteins (LITEZ), uses two light-inducible dimerizing proteins from Arabidopsis thaliana, GIGANTEA and the LOV domain of FKF1, to control synthetic zinc finger transcription factor activity in human cells. Activation of gene expression in human cells engineered with LITEZ was reversible and repeatable by modulating the duration of illumination. The level of gene expression could also be controlled by modulating light intensity. Finally, gene expression could be activated in a spatially defined pattern by illuminating the human cell culture through a photomask of arbitrary geometry. LITEZ enables new approaches for precisely regulating gene expression in biotechnology and medicine, as well as studying gene function, cell-cell interactions, and tissue morphogenesis.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

October 2012

Volume

134

Issue

40

Start / End Page

16480 / 16483

Related Subject Headings

  • Zinc Fingers
  • Transfection
  • Transcriptional Activation
  • Transcription Factors
  • Recombinant Fusion Proteins
  • Protein Engineering
  • Light
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

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ICMJE
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Polstein, L. R., & Gersbach, C. A. (2012). Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Journal of the American Chemical Society, 134(40), 16480–16483. https://doi.org/10.1021/ja3065667
Polstein, Lauren R., and Charles A. Gersbach. “Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors.Journal of the American Chemical Society 134, no. 40 (October 2012): 16480–83. https://doi.org/10.1021/ja3065667.
Polstein LR, Gersbach CA. Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Journal of the American Chemical Society. 2012 Oct;134(40):16480–3.
Polstein, Lauren R., and Charles A. Gersbach. “Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors.Journal of the American Chemical Society, vol. 134, no. 40, Oct. 2012, pp. 16480–83. Epmc, doi:10.1021/ja3065667.
Polstein LR, Gersbach CA. Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Journal of the American Chemical Society. 2012 Oct;134(40):16480–16483.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

October 2012

Volume

134

Issue

40

Start / End Page

16480 / 16483

Related Subject Headings

  • Zinc Fingers
  • Transfection
  • Transcriptional Activation
  • Transcription Factors
  • Recombinant Fusion Proteins
  • Protein Engineering
  • Light
  • Humans
  • Hela Cells
  • HeLa Cells