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Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile.

Publication ,  Journal Article
Wilson, KH; Kennedy, MJ; Fekety, FR
Published in: J Clin Microbiol
March 1982

Isolation of Clostridium difficile from fecal specimens has been facilitated by the development of a selective and differential medium, cefoxitin-cycloserinefructose agar (CCFA). We substituted 0.1% sodium taurocholate for the 2.5% egg yolk in CCFA and compared the growth of 15 isolates of C. difficile on the resulting medium with growth on conventional CCFA. The taurocholate-containing medium (TCCFA) quantitatively recovered vegetative forms of C. difficile in the same numbers as CCFA medium. Recovery of spores was a mean 1.7 log(10) higher on TCCFA than on CCFA. Thirty-six of 60 patient stool specimens growing C. difficile gave a heavier growth on TCCFA than on CCFA, and 9 failed to yield C. difficile on CCFA. TCCFA detected spores of 75 colony-forming units per ml from artificially inoculated fecal specimens when conventional stool culturing techniques were used. Fluorescence of colonies of C. difficile was more intense on TCCFA than on CCFA. TCCFA was simpler to prepare and, overall, was more sensitive than CCFA.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 1982

Volume

15

Issue

3

Start / End Page

443 / 446

Location

United States

Related Subject Headings

  • Taurocholic Acid
  • Spores, Bacterial
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Clostridium
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
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Wilson, K. H., Kennedy, M. J., & Fekety, F. R. (1982). Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J Clin Microbiol, 15(3), 443–446. https://doi.org/10.1128/jcm.15.3.443-446.1982
Wilson, K. H., M. J. Kennedy, and F. R. Fekety. “Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile.J Clin Microbiol 15, no. 3 (March 1982): 443–46. https://doi.org/10.1128/jcm.15.3.443-446.1982.
Wilson KH, Kennedy MJ, Fekety FR. Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J Clin Microbiol. 1982 Mar;15(3):443–6.
Wilson, K. H., et al. “Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile.J Clin Microbiol, vol. 15, no. 3, Mar. 1982, pp. 443–46. Pubmed, doi:10.1128/jcm.15.3.443-446.1982.
Wilson KH, Kennedy MJ, Fekety FR. Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J Clin Microbiol. 1982 Mar;15(3):443–446.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 1982

Volume

15

Issue

3

Start / End Page

443 / 446

Location

United States

Related Subject Headings

  • Taurocholic Acid
  • Spores, Bacterial
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Clostridium
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences