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Identification of a region of genetic variability among Bacillus anthracis strains and related species.

Publication ,  Journal Article
Andersen, GL; Simchock, JM; Wilson, KH
Published in: J Bacteriol
January 1996

The identification of a region of sequence variability among individual isolates of Bacillus anthracis as well as the two closely related species, Bacillus cereus and Bacillus mycoides, has made a sequence-based approach for the rapid differentiation among members of this group possible. We have identified this region of sequence divergence by comparison of arbitrarily primed (AP)-PCR "fingerprints" generated by an M13 bacteriophage-derived primer and sequencing the respective forms of the only polymorphic fragment observed. The 1,480-bp fragment derived from genomic DNA of the Sterne strain of B. anthracis contained four consecutive repeats of CAATATCAACAA. The same fragment from the Vollum strain was identical except that two of these repeats were deleted. The Ames strain of B. anthracis differed from the Sterne strain by a single-nucleotide deletion. More than 150 nucleotide differences separated B. cereus and B. mycoides from B. anthracis in pairwise comparisons. The nucleotide sequence of the variable fragment from each species contained one complete open reading frame (ORF) (designated vrrA, for variable region with repetitive sequence), encoding a potential 30-kDa protein located between the carboxy terminus of an upstream ORF (designated orf1) and the amino terminus of a downstream ORF (designated lytB). The sequence variation was primarily in vrrA, which was glutamine- and proline-rich (30% of total) and contained repetitive regions. A large proportion of the nucleotide substitutions between species were synonymous. vrrA has 35% identity with the microfilarial sheath protein shp2 of the parasitic worm Litomosoides carinii.

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Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1996

Volume

178

Issue

2

Start / End Page

377 / 384

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Repetitive Sequences, Nucleic Acid
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Open Reading Frames
  • Molecular Sequence Data
  • Microbiology
  • Genetic Variation
 

Citation

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Andersen, G. L., Simchock, J. M., & Wilson, K. H. (1996). Identification of a region of genetic variability among Bacillus anthracis strains and related species. J Bacteriol, 178(2), 377–384. https://doi.org/10.1128/jb.178.2.377-384.1996
Andersen, G. L., J. M. Simchock, and K. H. Wilson. “Identification of a region of genetic variability among Bacillus anthracis strains and related species.J Bacteriol 178, no. 2 (January 1996): 377–84. https://doi.org/10.1128/jb.178.2.377-384.1996.
Andersen GL, Simchock JM, Wilson KH. Identification of a region of genetic variability among Bacillus anthracis strains and related species. J Bacteriol. 1996 Jan;178(2):377–84.
Andersen, G. L., et al. “Identification of a region of genetic variability among Bacillus anthracis strains and related species.J Bacteriol, vol. 178, no. 2, Jan. 1996, pp. 377–84. Pubmed, doi:10.1128/jb.178.2.377-384.1996.
Andersen GL, Simchock JM, Wilson KH. Identification of a region of genetic variability among Bacillus anthracis strains and related species. J Bacteriol. 1996 Jan;178(2):377–384.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1996

Volume

178

Issue

2

Start / End Page

377 / 384

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Repetitive Sequences, Nucleic Acid
  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Open Reading Frames
  • Molecular Sequence Data
  • Microbiology
  • Genetic Variation