Scholars@Duke publication: Phylogeny of the Whipple's-disease-associated bacterium.

Phylogeny of the Whipple's-disease-associated bacterium.

Publication , Journal Article

Wilson, KH; Blitchington, R; Frothingham, R; Wilson, JA

Published in: Lancet

August 24, 1991

Efforts to culture and identify the intracellular bacteria associated with Whipple's disease have been unsuccessful. Nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 S ribosomal DNA present in a small-bowel biopsy specimen taken from a patient with Whipple's disease. A search by computer for similar rRNA sequences filed in databases showed the Whipple's-associated organism to be most similar to bacteria of the Rhodococcus, Streptomyces, and Arthrobacter genera, and more weakly related to mycobacteria. The biopsy specimen was estimated to contain around 10(7) cells of the organism. The probable aetiological agent for our patient's illness has not been identified previously in a patient with Whipple's disease.

Duke Scholars

Author Kenneth Heaton Wilson Medicine, Infectious Diseases

Author Richard Frothingham Medicine, Infectious Diseases

Author Joanne A. Peebles Wilson Medicine, Gastroenterology

Published In

Lancet

DOI

10.1016/0140-6736(91)90545-z

ISSN

0140-6736

Publication Date

August 24, 1991

Volume

338

Issue

8765

Start / End Page

474 / 475

Location

England

Related Subject Headings

Whipple Disease
RNA, Ribosomal, 16S
RNA, Bacterial
Phylogeny
Molecular Sequence Data
Intestine, Small
Intestinal Mucosa
Humans
Gram-Positive Bacteria
General & Internal Medicine

Citation

APA

Chicago

ICMJE

MLA

NLM

Wilson, K. H., Blitchington, R., Frothingham, R., & Wilson, J. A. (1991). Phylogeny of the Whipple's-disease-associated bacterium. Lancet, 338(8765), 474–475. https://doi.org/10.1016/0140-6736(91)90545-z

Wilson, K. H., R. Blitchington, R. Frothingham, and J. A. Wilson. “Phylogeny of the Whipple's-disease-associated bacterium.” Lancet 338, no. 8765 (August 24, 1991): 474–75. https://doi.org/10.1016/0140-6736(91)90545-z.

Wilson KH, Blitchington R, Frothingham R, Wilson JA. Phylogeny of the Whipple's-disease-associated bacterium. Lancet. 1991 Aug 24;338(8765):474–5.

Wilson, K. H., et al. “Phylogeny of the Whipple's-disease-associated bacterium.” Lancet, vol. 338, no. 8765, Aug. 1991, pp. 474–75. Pubmed, doi:10.1016/0140-6736(91)90545-z.

Wilson KH, Blitchington R, Frothingham R, Wilson JA. Phylogeny of the Whipple's-disease-associated bacterium. Lancet. 1991 Aug 24;338(8765):474–475.

Published In

Lancet

DOI

10.1016/0140-6736(91)90545-z

ISSN

0140-6736

Publication Date

August 24, 1991

Volume

338

Issue

8765

Start / End Page

474 / 475

Location

England

Related Subject Headings

Whipple Disease
RNA, Ribosomal, 16S
RNA, Bacterial
Phylogeny
Molecular Sequence Data
Intestine, Small
Intestinal Mucosa
Humans
Gram-Positive Bacteria
General & Internal Medicine