Skip to main content

Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study.

Publication ,  Journal Article
Denny, TN; Skurnick, JH; Garcia, A; Perez, G; Passannante, MR; Colon, J; Sheffet, A; Weiss, SH; Louria, D
Published in: Cytometry
March 15, 1996

The purpose of this study was to determine the types and distribution of immune subsets present in semen from human immunodeficiency virus (HIV)-infected (HIV+) individuals and to compare these values with those measures in semen from HIV-negative (HIV-) individuals. To accomplish this, a direct three-color monoclonal antibody labeling technique was employed to identify immune cells in fresh ejaculates. Once labeled, the percent of each immune subset present in the ejaculate was determined by flow cytometric analysis. The percent of CD3+ cells present in the semen of the HIV+ group showed no significant difference when compared with semen from the HIV- group. Analysis of the CD4+ subset yielded a significantly lower percent in the HIV+ group than in the HIV- group. The analysis of the CD8+ subset yielded a higher percent of cells present in semen from HIV+ individuals. The CD8 higher value along with lower CD4 value results in a lower CD4/CD8 ratio in the HIV+ group. Further subset studies showed that the percent of cells expressing naive (CD4+ CD45RA+) and memory (CD4+ CD45RO+) markers was lower in the HIV+ group. This study provides additional data supporting the utility of flow cytometry and monoclonal antibodies to immunophenotypic cells present in semen ejaculates. It is also the first reported application of the technique to a disease-based model and may be useful to better understand issues of mucosal immunity and transmission of sexually transmitted diseases such as HIV.

Duke Scholars

Published In

Cytometry

DOI

ISSN

0196-4763

Publication Date

March 15, 1996

Volume

26

Issue

1

Start / End Page

47 / 51

Location

United States

Related Subject Headings

  • Sexual Behavior
  • Semen
  • Male
  • Immunophenotyping
  • Humans
  • HIV Seropositivity
  • HIV Seronegativity
  • Flow Cytometry
  • CD4-CD8 Ratio
  • CD3 Complex
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Denny, T. N., Skurnick, J. H., Garcia, A., Perez, G., Passannante, M. R., Colon, J., … Louria, D. (1996). Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study. Cytometry, 26(1), 47–51. https://doi.org/10.1002/(SICI)1097-0320(19960315)26:1<47::AID-CYTO7>3.0.CO;2-D
Denny, T. N., J. H. Skurnick, A. Garcia, G. Perez, M. R. Passannante, J. Colon, A. Sheffet, S. H. Weiss, and D. Louria. “Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study.Cytometry 26, no. 1 (March 15, 1996): 47–51. https://doi.org/10.1002/(SICI)1097-0320(19960315)26:1<47::AID-CYTO7>3.0.CO;2-D.
Denny TN, Skurnick JH, Garcia A, Perez G, Passannante MR, Colon J, et al. Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study. Cytometry. 1996 Mar 15;26(1):47–51.
Denny, T. N., et al. “Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study.Cytometry, vol. 26, no. 1, Mar. 1996, pp. 47–51. Pubmed, doi:10.1002/(SICI)1097-0320(19960315)26:1<47::AID-CYTO7>3.0.CO;2-D.
Denny TN, Skurnick JH, Garcia A, Perez G, Passannante MR, Colon J, Sheffet A, Weiss SH, Louria D. Lymphocyte immunoregulatory cells present in semen from human immunodeficiency virus (HIV)-infected individuals: a report from the HIV Heterosexual Transmission Study. Cytometry. 1996 Mar 15;26(1):47–51.

Published In

Cytometry

DOI

ISSN

0196-4763

Publication Date

March 15, 1996

Volume

26

Issue

1

Start / End Page

47 / 51

Location

United States

Related Subject Headings

  • Sexual Behavior
  • Semen
  • Male
  • Immunophenotyping
  • Humans
  • HIV Seropositivity
  • HIV Seronegativity
  • Flow Cytometry
  • CD4-CD8 Ratio
  • CD3 Complex