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Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics.

Publication ,  Journal Article
Stayton, PS; Hoffman, AS; Murthy, N; Lackey, C; Cheung, C; Tan, P; Klumb, LA; Chilkoti, A; Wilbur, FS; Press, OW
Published in: Journal of controlled release : official journal of the Controlled Release Society
March 2000

There are many protein and DNA based therapeutics under development in the biotechnology and pharmaceutical industries. Key delivery challenges remain before many of these biomolecular therapeutics reach the clinic. Two important barriers are the effective targeting of drugs to specific tissues and cells and the subsequent intracellular delivery to appropriate cellular compartments. In this review, we summarize protein engineering work aimed at improving the stability and refolding efficiency of antibody fragments used in targeting, and at constructing new streptavidin variants which may offer improved performance in pre-targeting delivery strategies. In addition, we review recent work with pH-responsive polymers that mimic the membrane disruptive properties of viruses and toxins. These polymers could serve as alternatives to fusogenic peptides in gene therapy formulations and to enhance the intracellular delivery of protein therapeutics that function in the cytoplasm.

Duke Scholars

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Published In

Journal of controlled release : official journal of the Controlled Release Society

DOI

EISSN

1873-4995

ISSN

0168-3659

Publication Date

March 2000

Volume

65

Issue

1-2

Start / End Page

203 / 220

Related Subject Headings

  • Streptavidin
  • Protein Engineering
  • Protein Conformation
  • Polymers
  • Pharmacology & Pharmacy
  • Indicators and Reagents
  • Genetic Therapy
  • Drug Delivery Systems
  • Antibodies
  • 4003 Biomedical engineering
 

Citation

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Stayton, P. S., Hoffman, A. S., Murthy, N., Lackey, C., Cheung, C., Tan, P., … Press, O. W. (2000). Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. Journal of Controlled Release : Official Journal of the Controlled Release Society, 65(1–2), 203–220. https://doi.org/10.1016/s0168-3659(99)00236-9
Stayton, P. S., A. S. Hoffman, N. Murthy, C. Lackey, C. Cheung, P. Tan, L. A. Klumb, A. Chilkoti, F. S. Wilbur, and O. W. Press. “Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics.Journal of Controlled Release : Official Journal of the Controlled Release Society 65, no. 1–2 (March 2000): 203–20. https://doi.org/10.1016/s0168-3659(99)00236-9.
Stayton PS, Hoffman AS, Murthy N, Lackey C, Cheung C, Tan P, et al. Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. Journal of controlled release : official journal of the Controlled Release Society. 2000 Mar;65(1–2):203–20.
Stayton, P. S., et al. “Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics.Journal of Controlled Release : Official Journal of the Controlled Release Society, vol. 65, no. 1–2, Mar. 2000, pp. 203–20. Epmc, doi:10.1016/s0168-3659(99)00236-9.
Stayton PS, Hoffman AS, Murthy N, Lackey C, Cheung C, Tan P, Klumb LA, Chilkoti A, Wilbur FS, Press OW. Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. Journal of controlled release : official journal of the Controlled Release Society. 2000 Mar;65(1–2):203–220.
Journal cover image

Published In

Journal of controlled release : official journal of the Controlled Release Society

DOI

EISSN

1873-4995

ISSN

0168-3659

Publication Date

March 2000

Volume

65

Issue

1-2

Start / End Page

203 / 220

Related Subject Headings

  • Streptavidin
  • Protein Engineering
  • Protein Conformation
  • Polymers
  • Pharmacology & Pharmacy
  • Indicators and Reagents
  • Genetic Therapy
  • Drug Delivery Systems
  • Antibodies
  • 4003 Biomedical engineering