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Streptavidin-biotin binding energetics.

Publication ,  Journal Article
Stayton, PS; Freitag, S; Klumb, LA; Chilkoti, A; Chu, V; Penzotti, JE; To, R; Hyre, D; Le Trong, I; Lybrand, TP; Stenkamp, RE
Published in: Biomolecular engineering
December 1999

The high affinity energetics in the streptavidin-biotin system provide an excellent model system for studying how proteins balance enthalpic and entropic components to generate an impressive overall free energy for ligand binding. We review here concerted site-directed mutagenesis, biophysical, and computational studies of aromatic and hydrogen bonding interaction energetics between streptavidin and biotin. These results also have provided insight into how streptavidin builds a large activation barrier to dissociation by managing the enthalpic and entropic activation components. Finally, we review recent studies of the biotin dissociation pathway that address the fundamental question of how ligands exit protein binding pockets.

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Published In

Biomolecular engineering

DOI

ISSN

1389-0344

Publication Date

December 1999

Volume

16

Issue

1-4

Start / End Page

39 / 44

Related Subject Headings

  • Thermodynamics
  • Streptavidin
  • Protein Engineering
  • Protein Conformation
  • Protein Binding
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Ligands
  • Biotin
  • Biotechnology
 

Citation

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ICMJE
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Stayton, P. S., Freitag, S., Klumb, L. A., Chilkoti, A., Chu, V., Penzotti, J. E., … Stenkamp, R. E. (1999). Streptavidin-biotin binding energetics. Biomolecular Engineering, 16(1–4), 39–44. https://doi.org/10.1016/s1050-3862(99)00042-x
Stayton, P. S., S. Freitag, L. A. Klumb, A. Chilkoti, V. Chu, J. E. Penzotti, R. To, et al. “Streptavidin-biotin binding energetics.Biomolecular Engineering 16, no. 1–4 (December 1999): 39–44. https://doi.org/10.1016/s1050-3862(99)00042-x.
Stayton PS, Freitag S, Klumb LA, Chilkoti A, Chu V, Penzotti JE, et al. Streptavidin-biotin binding energetics. Biomolecular engineering. 1999 Dec;16(1–4):39–44.
Stayton, P. S., et al. “Streptavidin-biotin binding energetics.Biomolecular Engineering, vol. 16, no. 1–4, Dec. 1999, pp. 39–44. Epmc, doi:10.1016/s1050-3862(99)00042-x.
Stayton PS, Freitag S, Klumb LA, Chilkoti A, Chu V, Penzotti JE, To R, Hyre D, Le Trong I, Lybrand TP, Stenkamp RE. Streptavidin-biotin binding energetics. Biomolecular engineering. 1999 Dec;16(1–4):39–44.
Journal cover image

Published In

Biomolecular engineering

DOI

ISSN

1389-0344

Publication Date

December 1999

Volume

16

Issue

1-4

Start / End Page

39 / 44

Related Subject Headings

  • Thermodynamics
  • Streptavidin
  • Protein Engineering
  • Protein Conformation
  • Protein Binding
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Ligands
  • Biotin
  • Biotechnology