Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Direct force measurements of the streptavidin-biotin interaction.

Publication ,  Journal Article
Wong, J; Chilkoti, A; Moy, VT
Published in: Biomolecular engineering
December 1999

The interaction between streptavidin and its ligand, biotin, were studied by direct force measurements. The complimentary approaches of surface force apparatus (SFA) and atomic force microscopy (AFM) were used to elucidate both long-range and short-range adhesive interactions of the streptavidin biotin interaction. The high spatial resolution of the SFA provided a detailed profile of the intersurface forces of apposing surfaces functionalized with streptavidin and biotin. Measurements obtained by the SFA corresponded to long and intermediate-range forces that are important in determining ligand receptor association. AFM was used to measure the unbinding force of individual streptavidin biotin complexes. These measurements revealed the short-range interactions (i.e. hydrophobic and hydrogen bonding forces) that stabilize the intermolecular bond.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biomolecular engineering

DOI

ISSN

1389-0344

Publication Date

December 1999

Volume

16

Issue

1-4

Start / End Page

45 / 55

Related Subject Headings

  • Thermodynamics
  • Surface Properties
  • Streptavidin
  • Protein Engineering
  • Protein Binding
  • Microscopy, Atomic Force
  • Ligands
  • Hydrogen Bonding
  • Biotin
  • Biotechnology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wong, J., Chilkoti, A., & Moy, V. T. (1999). Direct force measurements of the streptavidin-biotin interaction. Biomolecular Engineering, 16(1–4), 45–55. https://doi.org/10.1016/s1050-3862(99)00035-2
Wong, J., A. Chilkoti, and V. T. Moy. “Direct force measurements of the streptavidin-biotin interaction.Biomolecular Engineering 16, no. 1–4 (December 1999): 45–55. https://doi.org/10.1016/s1050-3862(99)00035-2.
Wong J, Chilkoti A, Moy VT. Direct force measurements of the streptavidin-biotin interaction. Biomolecular engineering. 1999 Dec;16(1–4):45–55.
Wong, J., et al. “Direct force measurements of the streptavidin-biotin interaction.Biomolecular Engineering, vol. 16, no. 1–4, Dec. 1999, pp. 45–55. Epmc, doi:10.1016/s1050-3862(99)00035-2.
Wong J, Chilkoti A, Moy VT. Direct force measurements of the streptavidin-biotin interaction. Biomolecular engineering. 1999 Dec;16(1–4):45–55.
Journal cover image

Published In

Biomolecular engineering

DOI

ISSN

1389-0344

Publication Date

December 1999

Volume

16

Issue

1-4

Start / End Page

45 / 55

Related Subject Headings

  • Thermodynamics
  • Surface Properties
  • Streptavidin
  • Protein Engineering
  • Protein Binding
  • Microscopy, Atomic Force
  • Ligands
  • Hydrogen Bonding
  • Biotin
  • Biotechnology