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Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development.

Publication ,  Journal Article
Diehl, AM; Michaelson, P; Yang, SQ
Published in: Gastroenterology
June 1994

BACKGROUND/AIMS: Recent evidence suggests that CCAAT/enhancer binding protein (C/EBP) transcription factors may regulate hepatocyte terminal differentiation. METHODS: To explore this possibility, the present study looked for variations in the expression or DNA binding activity of different C/EBP isoforms during rat postnatal liver development and determined which of the C/EBPs were expressed by adult hepatocytes in primary culture. RESULTS: In intact rats, hepatocyte proliferation is active for 2-3 weeks after birth. During this period of postnatal liver growth, several liver-specific functions emerge and C/EBP alpha, beta, and delta isoforms are induced. Nuclear expression of the 36-kilodalton C/EBP delta protein increases immediately after birth, followed first by increases in the 38-kilodalton C/EBP beta protein expression and then by increases in the 42-kilodalton C/EBP alpha protein expression. Changes in C/EBP DNA binding activity accompany developmental increases in C/EBP proteins. Messenger RNAs of all three C/EBP isoforms are expressed by mature hepatocytes in primary culture. CONCLUSIONS: Specific C/EBP isoforms are induced differentially during the course of rat postnatal liver development. Young adult rats and cultured adult hepatocytes express all three C/EBP isoforms. These results are consistent with (but do not prove) the theory that variations in C/EBP expression and function help regulate hepatocyte terminal differentiation.

Duke Scholars

Published In

Gastroenterology

DOI

ISSN

0016-5085

Publication Date

June 1994

Volume

106

Issue

6

Start / End Page

1625 / 1637

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Proliferating Cell Nuclear Antigen
  • Phosphoenolpyruvate Carboxykinase (GTP)
  • Nuclear Proteins
  • Liver
  • Isomerism
  • Gastroenterology & Hepatology
  • Fetus
 

Citation

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Diehl, A. M., Michaelson, P., & Yang, S. Q. (1994). Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development. Gastroenterology, 106(6), 1625–1637. https://doi.org/10.1016/0016-5085(94)90420-0
Diehl, A. M., P. Michaelson, and S. Q. Yang. “Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development.Gastroenterology 106, no. 6 (June 1994): 1625–37. https://doi.org/10.1016/0016-5085(94)90420-0.
Diehl AM, Michaelson P, Yang SQ. Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development. Gastroenterology. 1994 Jun;106(6):1625–37.
Diehl, A. M., et al. “Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development.Gastroenterology, vol. 106, no. 6, June 1994, pp. 1625–37. Pubmed, doi:10.1016/0016-5085(94)90420-0.
Diehl AM, Michaelson P, Yang SQ. Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development. Gastroenterology. 1994 Jun;106(6):1625–1637.
Journal cover image

Published In

Gastroenterology

DOI

ISSN

0016-5085

Publication Date

June 1994

Volume

106

Issue

6

Start / End Page

1625 / 1637

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Proliferating Cell Nuclear Antigen
  • Phosphoenolpyruvate Carboxykinase (GTP)
  • Nuclear Proteins
  • Liver
  • Isomerism
  • Gastroenterology & Hepatology
  • Fetus