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Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development.

Publication ,  Journal Article
Ton, C; Stamatiou, D; Dzau, VJ; Liew, C-C
Published in: Biochem Biophys Res Commun
September 6, 2002

Vertebrate embryogenesis is a complex process controlled by a transcriptional hierarchy that coordinates the action of thousands of genes. To identify and analyze the expression patterns of these genes, we constructed a zebrafish cDNA microarray containing 4512 unique genes identified from zebrafish embryonic heart, adult hearts, and skeletal muscle cDNA libraries. We examined the patterns of gene expression during development in the zebrafish between five time points relative to 12h post-fertilization (hpf). Differentially expressed genes can be grouped into two categories, early genes that are expressed at 5hpf and genes expressed at 48/72/120hpf. Furthermore, we report the utilization of cDNA microarray technology to investigate the adaptive molecular responses of zebrafish to hypoxia during development. Our study provides the first utilization of cDNA microarray in the zebrafish and reveals dynamic changes in levels of gene expression in relation to development and survival of the zebrafish embryos under hypoxic stress.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 6, 2002

Volume

296

Issue

5

Start / End Page

1134 / 1142

Location

United States

Related Subject Headings

  • Zebrafish
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Oligonucleotide Array Sequence Analysis
  • Kinetics
  • Gene Expression Profiling
  • Embryo, Nonmammalian
  • Cells, Cultured
  • Cell Hypoxia
  • Biochemistry & Molecular Biology
 

Citation

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MLA
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Ton, C., Stamatiou, D., Dzau, V. J., & Liew, C.-C. (2002). Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development. Biochem Biophys Res Commun, 296(5), 1134–1142. https://doi.org/10.1016/s0006-291x(02)02010-7
Ton, Christopher, Dimitri Stamatiou, Victor J. Dzau, and Choong-Chin Liew. “Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development.Biochem Biophys Res Commun 296, no. 5 (September 6, 2002): 1134–42. https://doi.org/10.1016/s0006-291x(02)02010-7.
Ton C, Stamatiou D, Dzau VJ, Liew C-C. Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development. Biochem Biophys Res Commun. 2002 Sep 6;296(5):1134–42.
Ton, Christopher, et al. “Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development.Biochem Biophys Res Commun, vol. 296, no. 5, Sept. 2002, pp. 1134–42. Pubmed, doi:10.1016/s0006-291x(02)02010-7.
Ton C, Stamatiou D, Dzau VJ, Liew C-C. Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development. Biochem Biophys Res Commun. 2002 Sep 6;296(5):1134–1142.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 6, 2002

Volume

296

Issue

5

Start / End Page

1134 / 1142

Location

United States

Related Subject Headings

  • Zebrafish
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Oligonucleotide Array Sequence Analysis
  • Kinetics
  • Gene Expression Profiling
  • Embryo, Nonmammalian
  • Cells, Cultured
  • Cell Hypoxia
  • Biochemistry & Molecular Biology