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Balloon catheterization induced arterial expression of embryonic fibronectins.

Publication ,  Journal Article
Dubin, D; Peters, JH; Brown, LF; Logan, B; Kent, KC; Berse, B; Berven, S; Cercek, B; Sharifi, BG; Pratt, RE
Published in: Arterioscler Thromb Vasc Biol
November 1995

Fibronectins (FNs) comprise a family of adhesive extracellular matrix proteins that arise by alternative splicing in three regions: V (IIICS), EIIIA (ED-A), and EIIIB (ED-B). FNs bearing the EIIIA and EIIIB segments are prevalent during embryogenesis, expressed to lesser degrees in normal adult tissues, and may be locally reexpressed at adult tissue injury. RNase mapping shows that normal rat arteries express low levels of FNs that are predominantly EIIIA- and EIIIB-. Following balloon injury, arterial walls produce increased total levels of FN transcripts that preferentially include both the EIIIA and EIIIB segments. However, despite inducing increased total FN mRNA, balloon injury does not alter the relative composition of V120+, V95+, AND V0 spliced forms. In situ hybridization reveals that as early as 4 days after injury medial cells express increased total FN mRNA, and by 7 days substantial neointimal and focal medial synthesis of EIIIA+, EIIIB+, and V120+ FNs occurs; macrophages do not significantly contribute to this observed vascular FN synthesis. Consistent with the mRNA data, immunofluorescence microscopic analysis reveals increased deposition of EIIIB+ and V+ FN protein forms in injured arterial walls, particularly within the neointima. Our results suggest that local synthesis of specific FN isoforms is important to the neointimal formation that ensues after balloon injury.

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Published In

Arterioscler Thromb Vasc Biol

DOI

ISSN

1079-5642

Publication Date

November 1995

Volume

15

Issue

11

Start / End Page

1958 / 1967

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA
  • Molecular Sequence Data
  • Male
  • In Situ Hybridization
  • Hyperplasia
  • Gene Expression Regulation, Developmental
  • Fibronectins
  • Extracellular Matrix
 

Citation

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Dubin, D., Peters, J. H., Brown, L. F., Logan, B., Kent, K. C., Berse, B., … Pratt, R. E. (1995). Balloon catheterization induced arterial expression of embryonic fibronectins. Arterioscler Thromb Vasc Biol, 15(11), 1958–1967. https://doi.org/10.1161/01.atv.15.11.1958
Dubin, D., J. H. Peters, L. F. Brown, B. Logan, K. C. Kent, B. Berse, S. Berven, B. Cercek, B. G. Sharifi, and R. E. Pratt. “Balloon catheterization induced arterial expression of embryonic fibronectins.Arterioscler Thromb Vasc Biol 15, no. 11 (November 1995): 1958–67. https://doi.org/10.1161/01.atv.15.11.1958.
Dubin D, Peters JH, Brown LF, Logan B, Kent KC, Berse B, et al. Balloon catheterization induced arterial expression of embryonic fibronectins. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):1958–67.
Dubin, D., et al. “Balloon catheterization induced arterial expression of embryonic fibronectins.Arterioscler Thromb Vasc Biol, vol. 15, no. 11, Nov. 1995, pp. 1958–67. Pubmed, doi:10.1161/01.atv.15.11.1958.
Dubin D, Peters JH, Brown LF, Logan B, Kent KC, Berse B, Berven S, Cercek B, Sharifi BG, Pratt RE. Balloon catheterization induced arterial expression of embryonic fibronectins. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):1958–1967.

Published In

Arterioscler Thromb Vasc Biol

DOI

ISSN

1079-5642

Publication Date

November 1995

Volume

15

Issue

11

Start / End Page

1958 / 1967

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA
  • Molecular Sequence Data
  • Male
  • In Situ Hybridization
  • Hyperplasia
  • Gene Expression Regulation, Developmental
  • Fibronectins
  • Extracellular Matrix