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Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme.

Publication ,  Journal Article
Koike, G; Krieger, JE; Jacob, HJ; Mukoyama, M; Pratt, RE; Dzau, VJ
Published in: Biochem Biophys Res Commun
January 14, 1994

Using genetic mapping approaches, a gene on chromosome 10, Bp1, has been identified in the stroke-prone spontaneously hypertensive rat (SHRSP) in the same region that contains the gene for angiotensin converting enzyme (ACE). Since ACE plays an important role in blood pressure regulation, the ACE gene is a leading candidate for Bp1. To examine the possibility that a structural abnormality of ACE exists in the SHRSP, we cloned and characterized the cDNAs for the Wistar-Kyoto rat (WKY) and SHRSP ACE. Both cDNAs encode a single polypeptide of 1,313 amino acid residues with an estimated molecular weight of 150.9 KDa. Five nucleotide differences were identified between the WKY and the SHRSP ACE cDNAs. One of these differences resulted in an amino acid substitution (Lys-207 in the WKY to Arg-207 in the SHRSP). But the enzymatic properties of partially purified ACE from the two strains were similar. Thus the data suggest that an alteration in the primary structure of rat ACE does not contribute to the hypertension in the SHRSP.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

January 14, 1994

Volume

198

Issue

1

Start / End Page

380 / 386

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Restriction Mapping
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Polymerase Chain Reaction
  • Peptidyl-Dipeptidase A
  • Molecular Sequence Data
  • Mice
 

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Koike, G., Krieger, J. E., Jacob, H. J., Mukoyama, M., Pratt, R. E., & Dzau, V. J. (1994). Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme. Biochem Biophys Res Commun, 198(1), 380–386. https://doi.org/10.1006/bbrc.1994.1053
Koike, G., J. E. Krieger, H. J. Jacob, M. Mukoyama, R. E. Pratt, and V. J. Dzau. “Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme.Biochem Biophys Res Commun 198, no. 1 (January 14, 1994): 380–86. https://doi.org/10.1006/bbrc.1994.1053.
Koike G, Krieger JE, Jacob HJ, Mukoyama M, Pratt RE, Dzau VJ. Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme. Biochem Biophys Res Commun. 1994 Jan 14;198(1):380–6.
Koike, G., et al. “Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme.Biochem Biophys Res Commun, vol. 198, no. 1, Jan. 1994, pp. 380–86. Pubmed, doi:10.1006/bbrc.1994.1053.
Koike G, Krieger JE, Jacob HJ, Mukoyama M, Pratt RE, Dzau VJ. Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme. Biochem Biophys Res Commun. 1994 Jan 14;198(1):380–386.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

January 14, 1994

Volume

198

Issue

1

Start / End Page

380 / 386

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Restriction Mapping
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Polymerase Chain Reaction
  • Peptidyl-Dipeptidase A
  • Molecular Sequence Data
  • Mice