Studies of the biosynthesis of renin with a cell-free translation system.
1. Poly(A)+ mRNA from mouse submaxillary gland encodes a polypeptide of molecular weight 48 000 (48K polypeptide) which is abundant in the male. 2. This polypeptide is selectively absent in the translation products of mRNA from a strain of genetically renin-deficient mice C57 BL/10J. 3. The 48K polypeptide binds and co-elutes in identical fashion with pure authentic renin on pepstatin affinity chromatography. 4. Immunoprecipitation of translation products of male glandular mRNA with renin-specific antibody yielded this 48K band upon analysis by SDS/polyacrylamide gel electrophoresis and fluorography. Pure renin of molecular weight 37 000 blocked the binding of this polypeptide to antirenin antibody. 5. Mouse submaxillary gland synthesizes a renin precursor. The renin mRNA is androgenically regulated.
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Related Subject Headings
- Submandibular Gland
- Renin
- Protein Biosynthesis
- Mice
- Cell-Free System
- Cardiovascular System & Hematology
- Animals
- 42 Health sciences
- 32 Biomedical and clinical sciences
- 11 Medical and Health Sciences
Citation
Published In
DOI
ISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Submandibular Gland
- Renin
- Protein Biosynthesis
- Mice
- Cell-Free System
- Cardiovascular System & Hematology
- Animals
- 42 Health sciences
- 32 Biomedical and clinical sciences
- 11 Medical and Health Sciences