Scholars@Duke publication: Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.

Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.

Publication , Journal Article

Ji, RR; Zhang, Q; Bedecs, K; Arvidsson, J; Zhang, X; Xu, XJ; Wiesenfeld-Hallin, Z; Bartfai, T; Hökfelt, T

Published in: Proc Natl Acad Sci U S A

December 20, 1994

Antisense (AS) oligonucleotides (ONs) to galanin (GAL) were applied to the proximal end of a transected sciatic nerve, allowing their cellular uptake and transport into injured axons. GAL expression in dorsal root ganglia and self-mutilation behavior (autotomy) were then studied. AS-ONs with phosphorothioate or allyl modifications significantly suppressed the axotomy-induced increase in GAL levels, as demonstrated by immunohistochemistry and exaggerated autotomy behavior, whereas no significant effect on GAL mRNA levels could be demonstrated with in situ hybridization. Allyl-ONs were more effective than phosphorothioate-ONs. An AS-ON with three base mismatches did not induce any of the above effects. These results support the view that the inhibition of axotomy-induced GAL up-regulation is related to autotomy.

Duke Scholars

Author Qin Zhang

Author Ru-Rong Ji Anesthesiology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.26.12540

ISSN

0027-8424

Publication Date

December 20, 1994

Volume

Issue

Start / End Page

12540 / 12543

Location

United States

Related Subject Headings

Self Mutilation
Rats, Sprague-Dawley
Rats
RNA, Messenger
Peptides
Oligonucleotides, Antisense
Neuronal Plasticity
Molecular Sequence Data
Gene Expression Regulation
Ganglia, Spinal

Citation

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Chicago

ICMJE

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NLM

Ji, R. R., Zhang, Q., Bedecs, K., Arvidsson, J., Zhang, X., Xu, X. J., … Hökfelt, T. (1994). Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy. Proc Natl Acad Sci U S A, 91(26), 12540–12543. https://doi.org/10.1073/pnas.91.26.12540

Ji, R. R., Q. Zhang, K. Bedecs, J. Arvidsson, X. Zhang, X. J. Xu, Z. Wiesenfeld-Hallin, T. Bartfai, and T. Hökfelt. “Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.” Proc Natl Acad Sci U S A 91, no. 26 (December 20, 1994): 12540–43. https://doi.org/10.1073/pnas.91.26.12540.

Ji RR, Zhang Q, Bedecs K, Arvidsson J, Zhang X, Xu XJ, et al. Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12540–3.

Ji, R. R., et al. “Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.” Proc Natl Acad Sci U S A, vol. 91, no. 26, Dec. 1994, pp. 12540–43. Pubmed, doi:10.1073/pnas.91.26.12540.

Ji RR, Zhang Q, Bedecs K, Arvidsson J, Zhang X, Xu XJ, Wiesenfeld-Hallin Z, Bartfai T, Hökfelt T. Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12540–12543.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.91.26.12540

ISSN

0027-8424

Publication Date

December 20, 1994

Volume

Issue

Start / End Page

12540 / 12543

Location

United States

Related Subject Headings

Self Mutilation
Rats, Sprague-Dawley
Rats
RNA, Messenger
Peptides
Oligonucleotides, Antisense
Neuronal Plasticity
Molecular Sequence Data
Gene Expression Regulation
Ganglia, Spinal