Skip to main content

The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.

Publication ,  Journal Article
Lutz-Freyermuth, C; Keene, JD
Published in: Mol Cell Biol
July 1989

The site of interaction between human U1 RNA and one of its uniquely associated proteins, A, was examined with in vitro binding assays. The A protein bound directly to stem-loop II of U1 RNA in a region which exhibits sequence similarity to U2 RNA. The similarity with U2 RNA was in a region that has been shown to interact with a U2 RNA-associated protein. The A protein-binding site on U1 RNA overlapped a previously described epitope for an RNA-specific human autoantibody (S. L. Deutscher and J. D. Keene, Proc. Natl. Acad. Sci. USA 85:3299-3303, 1988), supporting the hypothesis that the anti-RNA antibody originated as an anti-idiotypic response to A protein-specific autoantibodies.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 1989

Volume

9

Issue

7

Start / End Page

2975 / 2982

Location

United States

Related Subject Headings

  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • RNA-Binding Proteins
  • Precipitin Tests
  • Nucleic Acid Conformation
  • Molecular Weight
  • Humans
  • Hela Cells
  • HeLa Cells
  • Genetic Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lutz-Freyermuth, C., & Keene, J. D. (1989). The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs. Mol Cell Biol, 9(7), 2975–2982. https://doi.org/10.1128/mcb.9.7.2975-2982.1989
Lutz-Freyermuth, C., and J. D. Keene. “The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.Mol Cell Biol 9, no. 7 (July 1989): 2975–82. https://doi.org/10.1128/mcb.9.7.2975-2982.1989.
Lutz-Freyermuth, C., and J. D. Keene. “The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.Mol Cell Biol, vol. 9, no. 7, July 1989, pp. 2975–82. Pubmed, doi:10.1128/mcb.9.7.2975-2982.1989.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 1989

Volume

9

Issue

7

Start / End Page

2975 / 2982

Location

United States

Related Subject Headings

  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins
  • RNA-Binding Proteins
  • Precipitin Tests
  • Nucleic Acid Conformation
  • Molecular Weight
  • Humans
  • Hela Cells
  • HeLa Cells
  • Genetic Techniques