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Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host.

Publication ,  Journal Article
Gaufin, T; Pattison, M; Gautam, R; Stoulig, C; Dufour, J; MacFarland, J; Mandell, D; Tatum, C; Marx, MH; Ribeiro, RM; Montefiori, D ...
Published in: J Virol
October 2009

Simian immunodeficiency virus (SIV)-infected African nonhuman primates do not progress to AIDS in spite of high and persistent viral loads (VLs). Some authors consider the high viral replication observed in chronic natural SIV infections to be due to lower anti-SIV antibody titers than those in rhesus macaques, suggesting a role of antibodies in controlling viral replication. We therefore investigated the impact of antibody responses on the outcome of acute and chronic SIVagm replication in African green monkeys (AGMs). Nine AGMs were infected with SIVagm.sab. Four AGMs were infused with 50 mg/kg of body weight anti-CD20 (rituximab; a gift from Genentech) every 21 days, starting from day -7 postinfection up to 184 days. The remaining AGMs were used as controls and received SIVagm only. Rituximab-treated AGMs were successfully depleted of CD20 cells in peripheral blood, lymph nodes (LNs), and intestine, as shown by the dynamics of CD20+ and CD79a+ cells. There was no significant difference in VLs between CD20-depleted AGMs and control monkeys: peak VLs ranged from 10(7) to 10(8) copies/ml; set-point values were 10(4) to 10(5) SIV RNA copies/ml. Levels of acute mucosal CD4+ T-cell depletion were similar for treated and nontreated animals. SIVagm seroconversion was delayed for the CD20-depleted AGMs compared to results for the controls. There was a significant difference in both the timing and magnitude of neutralizing antibody responses for CD20-depleted AGMs compared to results for controls. CD20 depletion significantly altered the histological structure of the germinal centers in the LNs and Peyer's patches. Our results, although obtained with a limited number of animals, suggest that humoral immune responses play only a minor role in the control of SIV viral replication during acute and chronic SIV infection in natural hosts.

Duke Scholars

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

October 2009

Volume

83

Issue

20

Start / End Page

10347 / 10357

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Rituximab
  • Monkey Diseases
  • Lymphocyte Depletion
  • Lymphocyte Activation
  • Immunologic Factors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gaufin, T., Pattison, M., Gautam, R., Stoulig, C., Dufour, J., MacFarland, J., … Pandrea, I. (2009). Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host. J Virol, 83(20), 10347–10357. https://doi.org/10.1128/JVI.00880-09
Gaufin, Thaidra, Melissa Pattison, Rajeev Gautam, Crystal Stoulig, Jason Dufour, Jeanne MacFarland, Daniel Mandell, et al. “Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host.J Virol 83, no. 20 (October 2009): 10347–57. https://doi.org/10.1128/JVI.00880-09.
Gaufin T, Pattison M, Gautam R, Stoulig C, Dufour J, MacFarland J, et al. Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host. J Virol. 2009 Oct;83(20):10347–57.
Gaufin, Thaidra, et al. “Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host.J Virol, vol. 83, no. 20, Oct. 2009, pp. 10347–57. Pubmed, doi:10.1128/JVI.00880-09.
Gaufin T, Pattison M, Gautam R, Stoulig C, Dufour J, MacFarland J, Mandell D, Tatum C, Marx MH, Ribeiro RM, Montefiori D, Apetrei C, Pandrea I. Effect of B-cell depletion on viral replication and clinical outcome of simian immunodeficiency virus infection in a natural host. J Virol. 2009 Oct;83(20):10347–10357.

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

October 2009

Volume

83

Issue

20

Start / End Page

10347 / 10357

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Rituximab
  • Monkey Diseases
  • Lymphocyte Depletion
  • Lymphocyte Activation
  • Immunologic Factors