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A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei.

Publication ,  Journal Article
Dresser, M; Pisetsky, D; Warren, R; McCarty, G; Moses, M
Published in: J Immunol Methods
November 23, 1987

A new method for the cytological analysis of antinuclear antibody binding offers several advantages over conventional techniques. Nuclei in meiosis, prepared by surface-spreading spermatocytes, provide a detailed examination of the constituents of the nucleus--euchromatin, heterochromatin, sex chromatin, nucleoli, centromeres, and dense patches that seem related to RNA metabolism--and each of these structures can be seen to change in morphology and antibody labeling during the course of meiotic prophase. Results using sera from humans and mice with autoimmune disease, and using several mouse monoclonal antibodies, demonstrate the potential of this method for clinical and research applications, both for more common antibody types and for those that bind epitopes which are unique to germ cells.

Duke Scholars

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

November 23, 1987

Volume

104

Issue

1-2

Start / End Page

111 / 121

Location

Netherlands

Related Subject Headings

  • Synaptonemal Complex
  • Mice
  • Meiosis
  • Immunology
  • Humans
  • Germ Cells
  • Fluorescent Antibody Technique
  • Cricetinae
  • Centromere
  • Cell Nucleus
 

Citation

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ICMJE
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Dresser, M., Pisetsky, D., Warren, R., McCarty, G., & Moses, M. (1987). A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei. J Immunol Methods, 104(1–2), 111–121. https://doi.org/10.1016/0022-1759(87)90494-7
Dresser, M., D. Pisetsky, R. Warren, G. McCarty, and M. Moses. “A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei.J Immunol Methods 104, no. 1–2 (November 23, 1987): 111–21. https://doi.org/10.1016/0022-1759(87)90494-7.
Dresser M, Pisetsky D, Warren R, McCarty G, Moses M. A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei. J Immunol Methods. 1987 Nov 23;104(1–2):111–21.
Dresser, M., et al. “A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei.J Immunol Methods, vol. 104, no. 1–2, Nov. 1987, pp. 111–21. Pubmed, doi:10.1016/0022-1759(87)90494-7.
Dresser M, Pisetsky D, Warren R, McCarty G, Moses M. A new method for the cytological analysis of autoantibody specificities using whole-mount, surface-spread meiotic nuclei. J Immunol Methods. 1987 Nov 23;104(1–2):111–121.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

November 23, 1987

Volume

104

Issue

1-2

Start / End Page

111 / 121

Location

Netherlands

Related Subject Headings

  • Synaptonemal Complex
  • Mice
  • Meiosis
  • Immunology
  • Humans
  • Germ Cells
  • Fluorescent Antibody Technique
  • Cricetinae
  • Centromere
  • Cell Nucleus