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The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors.

Publication ,  Journal Article
Morris, KV; Higgins, J; Shen, X; Stine, JT; Pedersen, NC
Published in: Virus Res
August 2003

Human herpesvirus type 8 vMIP-II has one of the broadest ranges of chemokine receptor binding and therefore a multiplicity of biologic effects, both immunologic and antiviral. These properties make vMIP-II an attractive effector gene to be expressed from gene therapy vectors. The present studies were concerned with both therapeutic approaches: (1) an anti-simian immunodeficiency virus (SIV) biologic, and (2) an effector gene in SIV-based vectors. Regarding its antiviral properties, vMIP-II expressed from bacteria and SIV-based vectors bound the surface of CEMx174 cells and specifically suppressed SIVmac251 infection. A CCR3 monoclonal antibody partially inhibited vMIP-II binding, suggesting that both SIVmac251 and vMIP-II utilize a similar CCR3-like receptor for CEMx174 cell binding. Replication competent SIV-based vectors containing forward and reverse vMIP-II produced neither identifiable vMIP-II nor virions for the first 21 days. Virus replication occurred after this period. Significant sequence alterations in the forward vMIP-II containing replication competent vector transcripts were responsible for the failure of vMIP-II expression. The genetic basis for the initial failure to replicate virus and its later restoration was not determined but appeared in the II-PIMv containing vectors to coincide with deletions and compensatory rearrangements in nef 3' of the polypurine tract. Cells transfected with SIVmac239Delta3DeltaLTR-vMIP-II vectors expressed biologically active vMIP-II that bound CEMx174 cells and suppressed SIVmac251 infection. These data suggest that replication defective SIV vectors expressing immunobiolgic genes such as vMIP-II may prove useful in gene therapies, particularly in augmenting immune responses in chronically infected individuals.

Duke Scholars

Published In

Virus Res

DOI

ISSN

0168-1702

Publication Date

August 2003

Volume

94

Issue

2

Start / End Page

103 / 112

Location

Netherlands

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Transfection
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Receptors, Chemokine
  • RNA, Viral
  • Molecular Sequence Data
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morris, K. V., Higgins, J., Shen, X., Stine, J. T., & Pedersen, N. C. (2003). The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors. Virus Res, 94(2), 103–112. https://doi.org/10.1016/s0168-1702(03)00138-2
Morris, Kevin V., Joanne Higgins, Xiaoyng Shen, Jonny T. Stine, and Niels C. Pedersen. “The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors.Virus Res 94, no. 2 (August 2003): 103–12. https://doi.org/10.1016/s0168-1702(03)00138-2.
Morris KV, Higgins J, Shen X, Stine JT, Pedersen NC. The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors. Virus Res. 2003 Aug;94(2):103–12.
Morris, Kevin V., et al. “The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors.Virus Res, vol. 94, no. 2, Aug. 2003, pp. 103–12. Pubmed, doi:10.1016/s0168-1702(03)00138-2.
Morris KV, Higgins J, Shen X, Stine JT, Pedersen NC. The effects of HHV-8 vMIP-II on SIVmac251 infection and replication competent and incompetent SIVmac239Delta3 vectors. Virus Res. 2003 Aug;94(2):103–112.
Journal cover image

Published In

Virus Res

DOI

ISSN

0168-1702

Publication Date

August 2003

Volume

94

Issue

2

Start / End Page

103 / 112

Location

Netherlands

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Transfection
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Receptors, Chemokine
  • RNA, Viral
  • Molecular Sequence Data
  • Humans