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Expression cloning and characterization of the TGF-beta type III receptor.

Publication ,  Journal Article
Wang, XF; Lin, HY; Ng-Eaton, E; Downward, J; Lodish, HF; Weinberg, RA
Published in: Cell
November 15, 1991

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

November 15, 1991

Volume

67

Issue

4

Start / End Page

797 / 805

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Transfection
  • Receptors, Transforming Growth Factor beta
  • Receptors, Cell Surface
  • Rats
  • RNA, Messenger
  • Proteoglycans
  • Molecular Sequence Data
  • Membrane Proteins
  • Membrane Glycoproteins
 

Citation

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Wang, X. F., Lin, H. Y., Ng-Eaton, E., Downward, J., Lodish, H. F., & Weinberg, R. A. (1991). Expression cloning and characterization of the TGF-beta type III receptor. Cell, 67(4), 797–805. https://doi.org/10.1016/0092-8674(91)90074-9
Wang, X. F., H. Y. Lin, E. Ng-Eaton, J. Downward, H. F. Lodish, and R. A. Weinberg. “Expression cloning and characterization of the TGF-beta type III receptor.Cell 67, no. 4 (November 15, 1991): 797–805. https://doi.org/10.1016/0092-8674(91)90074-9.
Wang XF, Lin HY, Ng-Eaton E, Downward J, Lodish HF, Weinberg RA. Expression cloning and characterization of the TGF-beta type III receptor. Cell. 1991 Nov 15;67(4):797–805.
Wang, X. F., et al. “Expression cloning and characterization of the TGF-beta type III receptor.Cell, vol. 67, no. 4, Nov. 1991, pp. 797–805. Pubmed, doi:10.1016/0092-8674(91)90074-9.
Wang XF, Lin HY, Ng-Eaton E, Downward J, Lodish HF, Weinberg RA. Expression cloning and characterization of the TGF-beta type III receptor. Cell. 1991 Nov 15;67(4):797–805.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

November 15, 1991

Volume

67

Issue

4

Start / End Page

797 / 805

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Transfection
  • Receptors, Transforming Growth Factor beta
  • Receptors, Cell Surface
  • Rats
  • RNA, Messenger
  • Proteoglycans
  • Molecular Sequence Data
  • Membrane Proteins
  • Membrane Glycoproteins