
Expression cloning and characterization of the TGF-beta type III receptor.
The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.
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Related Subject Headings
- Transforming Growth Factor beta
- Transfection
- Receptors, Transforming Growth Factor beta
- Receptors, Cell Surface
- Rats
- RNA, Messenger
- Proteoglycans
- Molecular Sequence Data
- Membrane Proteins
- Membrane Glycoproteins
Citation

Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transforming Growth Factor beta
- Transfection
- Receptors, Transforming Growth Factor beta
- Receptors, Cell Surface
- Rats
- RNA, Messenger
- Proteoglycans
- Molecular Sequence Data
- Membrane Proteins
- Membrane Glycoproteins