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Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.

Publication ,  Journal Article
Olafsen, T; Bruland, OS; Zalutsky, MR; Sandlie, I
Published in: Nucl Med Biol
August 1995

Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.

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Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

August 1995

Volume

22

Issue

6

Start / End Page

765 / 771

Location

United States

Related Subject Headings

  • Radioimmunodetection
  • Protein Conformation
  • Polymerase Chain Reaction
  • Osteosarcoma
  • Nuclear Medicine & Medical Imaging
  • Molecular Sequence Data
  • Models, Molecular
  • Lysine
  • Immunoglobulin Variable Region
  • Immunoglobulin Light Chains
 

Citation

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Olafsen, T., Bruland, O. S., Zalutsky, M. R., & Sandlie, I. (1995). Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling. Nucl Med Biol, 22(6), 765–771. https://doi.org/10.1016/0969-8051(95)00014-o
Olafsen, T., O. S. Bruland, M. R. Zalutsky, and I. Sandlie. “Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.Nucl Med Biol 22, no. 6 (August 1995): 765–71. https://doi.org/10.1016/0969-8051(95)00014-o.
Olafsen, T., et al. “Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.Nucl Med Biol, vol. 22, no. 6, Aug. 1995, pp. 765–71. Pubmed, doi:10.1016/0969-8051(95)00014-o.
Journal cover image

Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

August 1995

Volume

22

Issue

6

Start / End Page

765 / 771

Location

United States

Related Subject Headings

  • Radioimmunodetection
  • Protein Conformation
  • Polymerase Chain Reaction
  • Osteosarcoma
  • Nuclear Medicine & Medical Imaging
  • Molecular Sequence Data
  • Models, Molecular
  • Lysine
  • Immunoglobulin Variable Region
  • Immunoglobulin Light Chains