Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel

Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells.

Publication ,  Journal Article
Hayes, DF; Noska, MA; Kufe, DW; Zalutsky, MR
Published in: Int J Rad Appl Instrum B
1988

The murine monoclonal antibody (MAb) designated DF3 is an IgG1 prepared against a membrane enriched fraction of human breast carcinoma. MAb DF3 reacts with a family of large molecular weight glycoproteins expressed by 78% of breast cancer cells and 95% of epithelial ovarian cancer cells. Binding to the breast cancer cell line, MCF-7, of native MAb DF3 was compared to that of MAb DF3 exposed to different concentrations of Iodogen or Bolton-Hunter reagent. The amount of MAb DF3 required to obtain half-maximal binding (B1/2max) with MCF-7 extract for native MAb DF3 IgG was 180 ng, while the B1/2max for MAb DF3 IgG exposed to 1 and 10 micrograms Iodogen was 580 ng and 1800 mg, respectively. In contrast, the B1/2max for MAb DF3 IgG treated with Bolton-Hunter reagent was not different from that for native MAb DF3 IgG. Similar results were obtained with F(ab')2. Immunoreactive fractions for the 125I-labeled MAb DF3 were 0.13, 0.24 and 0.65 for IgG after exposure to 1 microgram Iodogen, 10 micrograms Iodogen and Bolton-Hunter, respectively. Immunoreactive fractions for F(ab')2 were 0.08, 0.08, and 0.53 for 1 and 10 micrograms Iodogen and Bolton-Hunter reagent, respectively. Association constants (Ka) were significantly higher for MAb DF3 IgG radioiodinated with Bolton-Hunter (3.97 +/- 0.38 x 10(8) M-1) than for IgG exposed to 1 microgram Iodogen (2.78 +/- 0.30 x 10(8) M-1) or 10 micrograms Iodogen (1.03 +/- 0.12 x 10(8) M-1). Similarly, the Ka ratio observed for the F(ab')2 radioiodinated with Bolton-Hunter reagent, 1 or 10 micrograms Iodogen was 22 to 3 to 1.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Int J Rad Appl Instrum B

DOI

ISSN

0883-2897

Publication Date

1988

Volume

15

Issue

3

Start / End Page

235 / 241

Location

England

Related Subject Headings

  • Radionuclide Imaging
  • Isotope Labeling
  • Iodine Radioisotopes
  • Humans
  • Female
  • Epitopes
  • Breast Neoplasms
  • Binding Sites, Antibody
  • Antigens, Tumor-Associated, Carbohydrate
  • Antigens, Neoplasm
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hayes, D. F., Noska, M. A., Kufe, D. W., & Zalutsky, M. R. (1988). Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells. Int J Rad Appl Instrum B, 15(3), 235–241. https://doi.org/10.1016/0883-2897(88)90101-8
Hayes, D. F., M. A. Noska, D. W. Kufe, and M. R. Zalutsky. “Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells.Int J Rad Appl Instrum B 15, no. 3 (1988): 235–41. https://doi.org/10.1016/0883-2897(88)90101-8.
Hayes DF, Noska MA, Kufe DW, Zalutsky MR. Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells. Int J Rad Appl Instrum B. 1988;15(3):235–41.
Hayes, D. F., et al. “Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells.Int J Rad Appl Instrum B, vol. 15, no. 3, 1988, pp. 235–41. Pubmed, doi:10.1016/0883-2897(88)90101-8.
Hayes DF, Noska MA, Kufe DW, Zalutsky MR. Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells. Int J Rad Appl Instrum B. 1988;15(3):235–241.

Published In

Int J Rad Appl Instrum B

DOI

ISSN

0883-2897

Publication Date

1988

Volume

15

Issue

3

Start / End Page

235 / 241

Location

England

Related Subject Headings

  • Radionuclide Imaging
  • Isotope Labeling
  • Iodine Radioisotopes
  • Humans
  • Female
  • Epitopes
  • Breast Neoplasms
  • Binding Sites, Antibody
  • Antigens, Tumor-Associated, Carbohydrate
  • Antigens, Neoplasm