A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine.
Publication
, Journal Article
Zalutsky, MR; Narula, AS
Published in: Int J Rad Appl Instrum A
1987
A procedure is described for the radioiodination of proteins using an iodinated derivative of N-succinimidyl 3-(tri-n-butylstannyl) benzoate (ATE). Adequate removal of unreacted ATE from [125I]ATE was necessary for optimal protein radioiodination. Labeling efficiencies of greater than 60% could be obtained after a 20 min incubation of goat IgG with [125I]ATE at 4 degrees C. Paired-label experiments with goat IgG labeled with 125I using ATE and 131I using Iodogen demonstrated that use of the ATE reagent for protein labeling significantly reduced (P less than 0.005) the thyroid uptake of radioiodine.
Duke Scholars
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Published In
Int J Rad Appl Instrum A
DOI
ISSN
0883-2889
Publication Date
1987
Volume
38
Issue
12
Start / End Page
1051 / 1055
Location
England
Related Subject Headings
- Urea
- Trialkyltin Compounds
- Tissue Distribution
- Thyroid Gland
- Succinimides
- Radionuclide Imaging
- Mice
- Isotope Labeling
- Iodine Radioisotopes
- Indicators and Reagents
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Zalutsky, M. R., & Narula, A. S. (1987). A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine. Int J Rad Appl Instrum A, 38(12), 1051–1055. https://doi.org/10.1016/0883-2889(87)90069-4
Zalutsky, M. R., and A. S. Narula. “A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine.” Int J Rad Appl Instrum A 38, no. 12 (1987): 1051–55. https://doi.org/10.1016/0883-2889(87)90069-4.
Zalutsky MR, Narula AS. A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine. Int J Rad Appl Instrum A. 1987;38(12):1051–5.
Zalutsky, M. R., and A. S. Narula. “A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine.” Int J Rad Appl Instrum A, vol. 38, no. 12, 1987, pp. 1051–55. Pubmed, doi:10.1016/0883-2889(87)90069-4.
Zalutsky MR, Narula AS. A method for the radiohalogenation of proteins resulting in decreased thyroid uptake of radioiodine. Int J Rad Appl Instrum A. 1987;38(12):1051–1055.
Published In
Int J Rad Appl Instrum A
DOI
ISSN
0883-2889
Publication Date
1987
Volume
38
Issue
12
Start / End Page
1051 / 1055
Location
England
Related Subject Headings
- Urea
- Trialkyltin Compounds
- Tissue Distribution
- Thyroid Gland
- Succinimides
- Radionuclide Imaging
- Mice
- Isotope Labeling
- Iodine Radioisotopes
- Indicators and Reagents