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Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity.

Publication ,  Journal Article
McDade, TW; Stallings, JF; Angold, A; Costello, EJ; Burleson, M; Cacioppo, JT; Glaser, R; Worthman, CM
Published in: Psychosom Med
2000

OBJECTIVE: Study 1: Introduce and validate a method for measuring EBV p18-VCA antibodies in whole blood spots to provide a minimally invasive marker of cell-mediated immune function. Study 2: Apply this method to a large community-based study of psychopathology in children and adolescents. METHODS: The EBV antibody method was evaluated through analysis of precision, reliability, stability, and comparisons with plasma and indirect immunofluorescence methods. The effects of life events on p18-VCA antibody level were considered in a subsample of 9, 11, and 13 year-old children participating in the Great Smoky Mountains Study in North Carolina. The subsample was stratified by age, sex, and degree of overall life strain. RESULTS: Dried blood spots provided a convenient, sensitive, precise, and reliable method for measuring EBV p18-VCA antibody titer. Life events were positively associated with p18-VCA antibodies in girls but not in boys. CONCLUSIONS: The validity of the blood spot EBV p18-VCA antibody assay, as well as the ease of sample collection, storage, and transportation, may provide an opportunity for psychoneuroimmunology to explore a wider range of stress models in larger, community-based studies.

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Published In

Psychosom Med

DOI

ISSN

0033-3174

Publication Date

2000

Volume

62

Issue

4

Start / End Page

560 / 567

Location

United States

Related Subject Headings

  • Sex Factors
  • Psychophysiologic Disorders
  • Psychoneuroimmunology
  • Psychiatry
  • Pilot Projects
  • Male
  • Life Change Events
  • Immunity, Cellular
  • Humans
  • Herpesvirus 4, Human
 

Citation

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McDade, T. W., Stallings, J. F., Angold, A., Costello, E. J., Burleson, M., Cacioppo, J. T., … Worthman, C. M. (2000). Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity. Psychosom Med, 62(4), 560–567. https://doi.org/10.1097/00006842-200007000-00015
McDade, T. W., J. F. Stallings, A. Angold, E. J. Costello, M. Burleson, J. T. Cacioppo, R. Glaser, and C. M. Worthman. “Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity.Psychosom Med 62, no. 4 (2000): 560–67. https://doi.org/10.1097/00006842-200007000-00015.
McDade TW, Stallings JF, Angold A, Costello EJ, Burleson M, Cacioppo JT, et al. Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity. Psychosom Med. 2000;62(4):560–7.
McDade, T. W., et al. “Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity.Psychosom Med, vol. 62, no. 4, 2000, pp. 560–67. Pubmed, doi:10.1097/00006842-200007000-00015.
McDade TW, Stallings JF, Angold A, Costello EJ, Burleson M, Cacioppo JT, Glaser R, Worthman CM. Epstein-Barr virus antibodies in whole blood spots: a minimally invasive method for assessing an aspect of cell-mediated immunity. Psychosom Med. 2000;62(4):560–567.

Published In

Psychosom Med

DOI

ISSN

0033-3174

Publication Date

2000

Volume

62

Issue

4

Start / End Page

560 / 567

Location

United States

Related Subject Headings

  • Sex Factors
  • Psychophysiologic Disorders
  • Psychoneuroimmunology
  • Psychiatry
  • Pilot Projects
  • Male
  • Life Change Events
  • Immunity, Cellular
  • Humans
  • Herpesvirus 4, Human