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Rvs161p and sphingolipids are required for actin repolarization following salt stress.

Publication ,  Journal Article
Balguerie, A; Bagnat, M; Bonneu, M; Aigle, M; Breton, AM
Published in: Eukaryot Cell
December 2002

In Saccharomyces cerevisiae, the actin cytoskeleton is depolarized by NaCl stress. In this study, the response was maximal after 30 min, and then actin patches repolarized. Rvs161p was required for actin repolarization because the rvs161delta mutant did not repolarize actin patches after growth in a salt medium. Mutations suppressing the rvs161delta-related salt sensitivity all occurred in genes required for sphingolipid biosynthesis: FEN1, SUR4, SUR2, SUR1, and IPT1. These suppressors also suppressed act1-1-related salt sensitivity and the defect in actin repolarization of the rvs161delta mutant, providing a link between sphingolipids and actin polarization. Indeed, deletion of the suppressor genes suppressed the rvs161delta defect in actin repolarization in two ways: either actin was not depolarized at the wild-type level in a set of suppressor mutants, or actin was repolarized in the absence of Rvs161p in the other suppressor mutants. Rvs161p was localized as cortical patches that concentrated at polarization sites, i.e., bud emergence and septa, and was found to be associated with lipid rafts. An important link between sphingolipids and actin polarization is that Rvs161p was required for actin repolarization and was found to be located in lipid rafts.

Duke Scholars

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

December 2002

Volume

1

Issue

6

Start / End Page

1021 / 1031

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Sphingolipids
  • Sodium Chloride
  • Salts
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Fusion Proteins
  • Plasmids
  • Mutation
 

Citation

APA
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MLA
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Balguerie, A., Bagnat, M., Bonneu, M., Aigle, M., & Breton, A. M. (2002). Rvs161p and sphingolipids are required for actin repolarization following salt stress. Eukaryot Cell, 1(6), 1021–1031. https://doi.org/10.1128/EC.1.6.1021-1031.2002
Balguerie, Axelle, Michel Bagnat, Marc Bonneu, Michel Aigle, and Annick M. Breton. “Rvs161p and sphingolipids are required for actin repolarization following salt stress.Eukaryot Cell 1, no. 6 (December 2002): 1021–31. https://doi.org/10.1128/EC.1.6.1021-1031.2002.
Balguerie A, Bagnat M, Bonneu M, Aigle M, Breton AM. Rvs161p and sphingolipids are required for actin repolarization following salt stress. Eukaryot Cell. 2002 Dec;1(6):1021–31.
Balguerie, Axelle, et al. “Rvs161p and sphingolipids are required for actin repolarization following salt stress.Eukaryot Cell, vol. 1, no. 6, Dec. 2002, pp. 1021–31. Pubmed, doi:10.1128/EC.1.6.1021-1031.2002.
Balguerie A, Bagnat M, Bonneu M, Aigle M, Breton AM. Rvs161p and sphingolipids are required for actin repolarization following salt stress. Eukaryot Cell. 2002 Dec;1(6):1021–1031.

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

December 2002

Volume

1

Issue

6

Start / End Page

1021 / 1031

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Sphingolipids
  • Sodium Chloride
  • Salts
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Fusion Proteins
  • Plasmids
  • Mutation