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Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer.

Publication ,  Journal Article
Maxwell, GL; Chandramouli, GVR; Dainty, L; Litzi, TJ; Berchuck, A; Barrett, JC; Risinger, JI
Published in: Clin Cancer Res
June 1, 2005

Previous studies using cDNA microarray have indicated that distinct gene expression profiles characterize endometrioid and papillary serous carcinomas of the endometrium. Molecular studies have observed that mixed mullerian tumors, characterized by both carcinomatous and sarcomatous components, share features that are characteristic of endometrial carcinomas. The objective of this analysis was to more precisely define gene expression patterns that distinguish endometrioid and papillary serous histologies of endometrial carcinoma and mixed mullerian tumors of the uterus. One hundred nineteen pathologically confirmed uterine cancer samples were studied (66 endometrioid, 24 papillary serous, and 29 mixed mullerian tumors). Gene expressions were analyzed using the Affymetrix Human Genome Arrays U133A and U133B Genechip set. Unsupervised analysis revealed distinct global gene expression patterns of endometrioid, papillary serous, mixed mullerian tumors, and normal tissues as grossly separated clusters. Two-sample t tests comparing endometrioid and papillary serous, endometrioid and mixed mullerian tumor, and papillary serous and mixed mullerian tumor pairs identified 1,055, 5,212, and 1,208 differentially expressed genes at P < 0.001, respectively. These data revealed that distinct patterns of gene expression characterize various histologic types of uterine cancer. Gene expression profiles for select genes were confirmed using quantitative PCR. An understanding of the molecular heterogeneity of various histologic types of endometrial cancer has the potential to lead to better individualization of treatment in the future.

Duke Scholars

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

June 1, 2005

Volume

11

Issue

11

Start / End Page

4056 / 4066

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Reproducibility of Results
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mixed Tumor, Mullerian
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Maxwell, G. L., Chandramouli, G. V. R., Dainty, L., Litzi, T. J., Berchuck, A., Barrett, J. C., & Risinger, J. I. (2005). Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer. Clin Cancer Res, 11(11), 4056–4066. https://doi.org/10.1158/1078-0432.CCR-04-2001
Maxwell, G Larry, G. V. R. Chandramouli, Lou Dainty, Tracy J. Litzi, Andrew Berchuck, J Carl Barrett, and John I. Risinger. “Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer.Clin Cancer Res 11, no. 11 (June 1, 2005): 4056–66. https://doi.org/10.1158/1078-0432.CCR-04-2001.
Maxwell GL, Chandramouli GVR, Dainty L, Litzi TJ, Berchuck A, Barrett JC, et al. Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer. Clin Cancer Res. 2005 Jun 1;11(11):4056–66.
Maxwell, G. Larry, et al. “Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer.Clin Cancer Res, vol. 11, no. 11, June 2005, pp. 4056–66. Pubmed, doi:10.1158/1078-0432.CCR-04-2001.
Maxwell GL, Chandramouli GVR, Dainty L, Litzi TJ, Berchuck A, Barrett JC, Risinger JI. Microarray analysis of endometrial carcinomas and mixed mullerian tumors reveals distinct gene expression profiles associated with different histologic types of uterine cancer. Clin Cancer Res. 2005 Jun 1;11(11):4056–4066.

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

June 1, 2005

Volume

11

Issue

11

Start / End Page

4056 / 4066

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Reproducibility of Results
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Mixed Tumor, Mullerian
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
  • Female