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Structure of the manganese-bound manganese transport regulator of Bacillus subtilis.

Publication ,  Journal Article
Glasfeld, A; Guedon, E; Helmann, JD; Brennan, RG
Published in: Nat Struct Biol
August 2003

The Bacillus subtilis manganese transport regulator, MntR, binds Mn2+ as an effector and is a repressor of transporters that import manganese. A member of the diphtheria toxin repressor (DtxR) family of metalloregulatory proteins, MntR exhibits selectivity for Mn2+ over Fe2+. Replacement of a metal-binding residue, Asp8, with methionine (D8M) relaxes this specificity. We report here the X-ray crystal structures of wild-type MntR and the D8M mutant bound to manganese with 1.75 A and 1.61 A resolution, respectively. The 142-residue MntR homodimer has substantial structural similarity to the 226-residue DtxR but lacks the C-terminal SH3-like domain of DtxR. The metal-binding pockets of MntR and DtxR are substantially different. The cation-to-cation distance between the two manganese ions bound by MntR is 3.3 A, whereas that between the metal ions bound by DtxR is 9 A. D8M binds only a single Mn2+ per monomer, owing to alteration of the metal-binding site. The sole retained metal site adopts pseudo-hexacoordinate geometry rather than the pseudo-heptacoordinate geometry of the MntR metal sites.

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Published In

Nat Struct Biol

DOI

ISSN

1072-8368

Publication Date

August 2003

Volume

10

Issue

8

Start / End Page

652 / 657

Location

United States

Related Subject Headings

  • Static Electricity
  • Sequence Homology, Amino Acid
  • Repressor Proteins
  • Recombinant Proteins
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Models, Molecular
  • Manganese
  • Developmental Biology
  • Crystallography, X-Ray
 

Citation

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Glasfeld, A., Guedon, E., Helmann, J. D., & Brennan, R. G. (2003). Structure of the manganese-bound manganese transport regulator of Bacillus subtilis. Nat Struct Biol, 10(8), 652–657. https://doi.org/10.1038/nsb951
Glasfeld, Arthur, Emmanuel Guedon, John D. Helmann, and Richard G. Brennan. “Structure of the manganese-bound manganese transport regulator of Bacillus subtilis.Nat Struct Biol 10, no. 8 (August 2003): 652–57. https://doi.org/10.1038/nsb951.
Glasfeld A, Guedon E, Helmann JD, Brennan RG. Structure of the manganese-bound manganese transport regulator of Bacillus subtilis. Nat Struct Biol. 2003 Aug;10(8):652–7.
Glasfeld, Arthur, et al. “Structure of the manganese-bound manganese transport regulator of Bacillus subtilis.Nat Struct Biol, vol. 10, no. 8, Aug. 2003, pp. 652–57. Pubmed, doi:10.1038/nsb951.
Glasfeld A, Guedon E, Helmann JD, Brennan RG. Structure of the manganese-bound manganese transport regulator of Bacillus subtilis. Nat Struct Biol. 2003 Aug;10(8):652–657.

Published In

Nat Struct Biol

DOI

ISSN

1072-8368

Publication Date

August 2003

Volume

10

Issue

8

Start / End Page

652 / 657

Location

United States

Related Subject Headings

  • Static Electricity
  • Sequence Homology, Amino Acid
  • Repressor Proteins
  • Recombinant Proteins
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Models, Molecular
  • Manganese
  • Developmental Biology
  • Crystallography, X-Ray