Skip to main content
Journal cover image

Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure.

Publication ,  Journal Article
Cho, E; Brown, A; Kuech, TF
Published in: Langmuir : the ACS journal of surfaces and colloids
August 2012

Single-stranded DNA immobilized on an III-V semiconductor is a potential high-sensitivity biosensor. The chemical and electronic changes occurring upon the binding of DNA to the InAs surface are essential to understanding the DNA-immobilization mechanism. In this work, the chemical properties of DNA-immobilized InAs surfaces were determined through high-resolution X-ray photoelectron spectroscopy (XPS) and near-edge X-ray absorption fine structure (NEXAFS). Prior to DNA functionalization, HF- and NH(4)OH- based aqueous etches were used to remove the native oxide from the InAs surface. The initial chemical state of the surface resulting from these etches were characterized prior to functionalization. F-tagged thiolated single-stranded DNA (ssDNA) was used as the probe species under two different functionalization methods. The presence of DNA immobilized on the surface was confirmed from the F 1s, N 1s, and P 2p peaks in the XPS spectra. The presence of salt had a profound effect on the density of immobilized DNA on the InAs surface. To study the interfacial chemistry, the surface was treated with thiolated ssDNA with and without the mercaptohexanol molecule. An analysis of the As 3d and In 3d spectra indicates that both In-S and As-S are present on the surface after DNA functionalization. The amount of In-S and As-S was determined by the functionalization method as well as the presence of mercaptohexanol during functionalization. The orientation of the adsorbed ssDNA is determined by polarization-dependent NEXAFS utilizing the N K-edge. The immobilized ssDNA molecule has a preferred tilt angle with respect to the substrate normal, but with a random azimuthal distribution.

Duke Scholars

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

August 2012

Volume

28

Issue

32

Start / End Page

11890 / 11898

Related Subject Headings

  • X-Ray Absorption Spectroscopy
  • Surface Properties
  • Photoelectron Spectroscopy
  • Oxides
  • Indium
  • DNA, Single-Stranded
  • DNA Probes
  • Chemical Physics
  • Base Sequence
  • Arsenicals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cho, E., Brown, A., & Kuech, T. F. (2012). Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure. Langmuir : The ACS Journal of Surfaces and Colloids, 28(32), 11890–11898. https://doi.org/10.1021/la302313v
Cho, EunKyung, April Brown, and Thomas F. Kuech. “Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure.Langmuir : The ACS Journal of Surfaces and Colloids 28, no. 32 (August 2012): 11890–98. https://doi.org/10.1021/la302313v.
Cho E, Brown A, Kuech TF. Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure. Langmuir : the ACS journal of surfaces and colloids. 2012 Aug;28(32):11890–8.
Cho, EunKyung, et al. “Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure.Langmuir : The ACS Journal of Surfaces and Colloids, vol. 28, no. 32, Aug. 2012, pp. 11890–98. Epmc, doi:10.1021/la302313v.
Cho E, Brown A, Kuech TF. Chemical characterization of DNA-immobilized InAs surfaces using X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure. Langmuir : the ACS journal of surfaces and colloids. 2012 Aug;28(32):11890–11898.
Journal cover image

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

August 2012

Volume

28

Issue

32

Start / End Page

11890 / 11898

Related Subject Headings

  • X-Ray Absorption Spectroscopy
  • Surface Properties
  • Photoelectron Spectroscopy
  • Oxides
  • Indium
  • DNA, Single-Stranded
  • DNA Probes
  • Chemical Physics
  • Base Sequence
  • Arsenicals