A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins
Publication
, Journal Article
Wang, MZ; Campa, M; Howard, BA; Patz, EF; Fitzgerald, MC
Published in: Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics
December 1, 2002
A protocol involving the combination of liquid-phase isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was developed. Liquid-phase isoelectric focusing was used to separate serum peptides and proteins into 20 different fractions based on their pl values using a Rotofor apparatus. A total of 2 ml of blood was subjected to fractionation. The results from replicate Rotofor runs indicate that the Rotofor fraction a protein appeared in varied from run to run.
Duke Scholars
Published In
Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
587 / 588
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Wang, M. Z., Campa, M., Howard, B. A., Patz, E. F., & Fitzgerald, M. C. (2002). A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, 587–588.
Wang, M. Z., M. Campa, B. A. Howard, E. F. Patz, and M. C. Fitzgerald. “A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins.” Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 587–88.
Wang MZ, Campa M, Howard BA, Patz EF, Fitzgerald MC. A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;587–8.
Wang, M. Z., et al. “A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins.” Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 587–88.
Wang MZ, Campa M, Howard BA, Patz EF, Fitzgerald MC. A fractionation protocol to reduce signal suppression effects in the MALDI analysis of blood proteins. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;587–588.
Published In
Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
587 / 588