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Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad.

Publication ,  Journal Article
Jameson, SA; Natarajan, A; Cool, J; DeFalco, T; Maatouk, DM; Mork, L; Munger, SC; Capel, B
Published in: PLoS Genet
2012

The divergence of distinct cell populations from multipotent progenitors is poorly understood, particularly in vivo. The gonad is an ideal place to study this process, because it originates as a bipotential primordium where multiple distinct lineages acquire sex-specific fates as the organ differentiates as a testis or an ovary. To gain a more detailed understanding of the process of gonadal differentiation at the level of the individual cell populations, we conducted microarrays on sorted cells from XX and XY mouse gonads at three time points spanning the period when the gonadal cells transition from sexually undifferentiated progenitors to their respective sex-specific fates. We analyzed supporting cells, interstitial/stromal cells, germ cells, and endothelial cells. This work identified genes specifically depleted and enriched in each lineage as it underwent sex-specific differentiation. We determined that the sexually undifferentiated germ cell and supporting cell progenitors showed lineage priming. We found that germ cell progenitors were primed with a bias toward the male fate. In contrast, supporting cells were primed with a female bias, indicative of the robust repression program involved in the commitment to XY supporting cell fate. This study provides a molecular explanation reconciling the female default and balanced models of sex determination and represents a rich resource for the field. More importantly, it yields new insights into the mechanisms by which different cell types in a single organ adopt their respective fates.

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Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

2012

Volume

8

Issue

3

Start / End Page

e1002575

Location

United States

Related Subject Headings

  • Stromal Cells
  • Sex Determination Processes
  • Microarray Analysis
  • Mice
  • Male
  • Gonads
  • Germ Cells
  • Gene Expression Regulation, Developmental
  • Female
  • Endothelial Cells
 

Citation

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Jameson, S. A., Natarajan, A., Cool, J., DeFalco, T., Maatouk, D. M., Mork, L., … Capel, B. (2012). Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad. PLoS Genet, 8(3), e1002575. https://doi.org/10.1371/journal.pgen.1002575
Jameson, Samantha A., Anirudh Natarajan, Jonah Cool, Tony DeFalco, Danielle M. Maatouk, Lindsey Mork, Steven C. Munger, and Blanche Capel. “Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad.PLoS Genet 8, no. 3 (2012): e1002575. https://doi.org/10.1371/journal.pgen.1002575.
Jameson SA, Natarajan A, Cool J, DeFalco T, Maatouk DM, Mork L, et al. Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad. PLoS Genet. 2012;8(3):e1002575.
Jameson, Samantha A., et al. “Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad.PLoS Genet, vol. 8, no. 3, 2012, p. e1002575. Pubmed, doi:10.1371/journal.pgen.1002575.
Jameson SA, Natarajan A, Cool J, DeFalco T, Maatouk DM, Mork L, Munger SC, Capel B. Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad. PLoS Genet. 2012;8(3):e1002575.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

2012

Volume

8

Issue

3

Start / End Page

e1002575

Location

United States

Related Subject Headings

  • Stromal Cells
  • Sex Determination Processes
  • Microarray Analysis
  • Mice
  • Male
  • Gonads
  • Germ Cells
  • Gene Expression Regulation, Developmental
  • Female
  • Endothelial Cells