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Sex chromatin staining in amnion cells.

Publication ,  Journal Article
Capel, B; Batchvarov, J
Published in: CSH Protoc
November 1, 2008

INTRODUCTIONThe procedure described here provides a quick and reliable method for determining the sex of mouse embryos that are <12.5 days post-coitum (dpc). Cells from amniotic membranes are stained with toluidine blue. The presence of a heavily stained condensed chromatin body (i.e., a Barr body) indicates the XX samples. With experience, we find a >95% concordance with genotyping data based on PCR for Y-chromosome sequences in extracted tail DNA. This protocol has the advantage of speed and efficiency: When assembling cultures with live tissue, samples can be sexed in 30 min.

Duke Scholars

Published In

CSH Protoc

DOI

Publication Date

November 1, 2008

Volume

2008

Start / End Page

pdb.prot5079

Location

United States

Related Subject Headings

  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)
 

Citation

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Capel, B., & Batchvarov, J. (2008). Sex chromatin staining in amnion cells. CSH Protoc, 2008, pdb.prot5079. https://doi.org/10.1101/pdb.prot5079
Capel, Blanche, and Jordan Batchvarov. “Sex chromatin staining in amnion cells.CSH Protoc 2008 (November 1, 2008): pdb.prot5079. https://doi.org/10.1101/pdb.prot5079.
Capel B, Batchvarov J. Sex chromatin staining in amnion cells. CSH Protoc. 2008 Nov 1;2008:pdb.prot5079.
Capel, Blanche, and Jordan Batchvarov. “Sex chromatin staining in amnion cells.CSH Protoc, vol. 2008, Nov. 2008, p. pdb.prot5079. Pubmed, doi:10.1101/pdb.prot5079.
Capel B, Batchvarov J. Sex chromatin staining in amnion cells. CSH Protoc. 2008 Nov 1;2008:pdb.prot5079.

Published In

CSH Protoc

DOI

Publication Date

November 1, 2008

Volume

2008

Start / End Page

pdb.prot5079

Location

United States

Related Subject Headings

  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)